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Monochlorobimane
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Monochlorobimane图片
CAS NO:76421-73-3
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议

产品名称
Chlorobimane
产品介绍
Monochlorobimane (Chlorobimane) 是一种荧光染料 (λex=380 nm,λem=470 nm),用于测定细胞中谷胱甘肽 (GSH)。
生物活性

Monochlorobimane (Chlorobimane) is afluorescent dye(λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of control and experimental wells:
    The experiment should consist of parallel negative, positive and experimental wells respectively.
    Experimental wells: add 200 μL of cell culture media containing your GSH effector of interest at desired concentration (e.g. 200 μM H2O2)
2. Incubate the plate overnight at 37 ℃, 5 % CO2. The incubation time varies depended on your normal protocol
3. Monochlorobimane (mBCl) is added to the cells at a final concentration of20-100 μMfrom a working solution of 1 mM. The stock solution of mBCl (50 mM) was prepared in dimethyl sulphoxide (DMSO) and stored at -20℃; the working solution was prepared before use by diluting the stock solution in 0.1 M PBS buffer (pH 7.0). In the assay the final concentration of DMSO was below 0.2% (v/v)
4. The cell suspensions were incubated with mBCl in the dark at 25℃ for ~2 h
5. Remove the dye and treatment by centrifugation at 700 X g for 5 min. Add 200 μL of the Assay Buffer to each well and continue to the preferred method of detection
6. Detection of intracellular GSH:
    Fluorescence plate reader: For adherent cells; read fluorescence directly off the culturing plate. If working with suspension cells; aliquot 200 μL of the culture suspension into the white opaque plate and record fluorescence at EX/EM= 380/465 ±20 nm respectively (blue).

分子量

226.66

性状

Solid

Formula

C10H11ClN2O2

CAS 号

76421-73-3

Emission (Em)
Em 450-495 nm Blue
Excitation (Ex)
Ex<380 nm Ultraviolet
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 50 mg/mL(220.59 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM4.4119 mL22.0595 mL44.1189 mL
5 mM0.8824 mL4.4119 mL8.8238 mL
10 mM0.4412 mL2.2059 mL4.4119 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: 2.5 mg/mL (11.03 mM); Clear solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (11.03 mM) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: 2.5 mg/mL (11.03 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (11.03 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在本网站选购。
染色示例
  • Description: Monochlorobimane (Chlorobimane) can be used to detect intracellular GSH levels.
    Method: For tissue labeling.
    1. Stain frozen sections of eyeballs with Monochlorobimane (30 min).
    2. Rinse and observe staining tissue under a fluorescence microscope.