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Lucigenin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Lucigenin图片
CAS NO:2315-97-1
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
50mg电议
100mg电议
500mg电议
1 g电议
5 g电议

产品名称
光泽精
NSC-151912
L-6868
产品介绍
Lucigenin(L-6868; NSC-151912)是一种化学发光探针用于检测内源性产生的超氧阴离子自由基在细胞的存在。
生物活性

Lucigenin(L-6868; NSC-151912) is a chemiluminescent probe used to indicate the presence of endogenously generated superoxide anion radicals in cells. Upon single-electron reduction of Lucigenin by a radical species the subsequent form of Lucigenin presents an amplified chemiluminescence which is measurable and can be correlated to the respective amount of reactive radical species present in the system. This approach can be used to probe the natural generation of these species as well as the natural functioning of superoxide dismutase and other enzymes responsible for scavenging radicals. In addition to indicating the presence of radicals in cells, Lucigenin shows interaction with redox-active oxidoreductase enzymes. Lucigenin is shown to mediate the reduction of cytochrome C byxanthine oxidaseand to increase the rate of NADPH oxidation. These observations indicate that use of Lucigenin for superoxide analysis is precluded in the presence of high levels of NAD(P)H-oxidoreductases.

分子量

510.50

性状

Solid

Formula

C28H22N4O6

CAS 号

2315-97-1

Emission (Em)
Em 495-570 nm Green
Excitation (Ex)
Ex<380 nm Ultraviolet
中文名称

光泽精

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO : 62.5 mg/mL(122.43 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.9589 mL9.7943 mL19.5886 mL
5 mM0.3918 mL1.9589 mL3.9177 mL
10 mM0.1959 mL0.9794 mL1.9589 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.08 mg/mL (4.07 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.07 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.08 mg/mL (4.07 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.07 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在本网站选购。
染色示例
  • Description: Lucigenin is a chemiluminescent probe, it can be used to detect superoxide anion radicals in cells under alkaline condition with green fluorescence.
    Method: For HCO3transport assay.
    1. Conidia and hyphae from the strains are treated at 28℃ with 0.4 M mannitol in a solution of 50 mM NaHCO3for 2 h.
    2. Wash cells and add 0.4 M mannitol to continue recovery (2 h; 28℃). A solution of only 0.4 M mannitol served as the control.
    3. Incubate strains with Lucigenin (10 mM) and observed with a fluorescence microscope.