Cresyl Violet acetate is a red fluorescent stain, which can be used to stain neurons.

The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm³and 1.82±0.33 mm³in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm³and 3740.1±784 μm³in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm³and 126.51±33 μm³in Cresyl Violet acetate staining and PV immunostaining sections, respectively^[1].

321.33

Solid

C₁₈H₁₅N₃O₃

10510-54-0

甲酚紫醋酸盐

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

DMSO : 1 mg/mL(3.11 mM;Need ultrasonic)

H₂O :< 0.1 mg/mL (ultrasonic;warming;heat to 60℃)(insoluble)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。