Thioflavine S is a fluorescent histochemical marker of dense core senile plaques.

Solid

1326-12-1

硫磺素；四羟酮醇 S；硫代黄色素 S；硫代磺素 S；硫磺素 S；直接黄 7

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

H₂O : ≥ 50 mg/mL

*"≥" means soluble, but saturation unknown.

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

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  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (Infinity mM); Clear solution; Need ultrasonic and warming and heat to 60℃

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  Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to measure the myocardial no-refow phenomenon.
  Method: For Thioflavine S staining assay.
  1. Thioflavine S (1 mL/kg, 6%) is injected into mice via the tail vein and allowed to circulate for 1-2 min.
  2. The hearts are quickly harvested, fixed in 4% paraformaldehyde (PFA) overnight, cut into 1 mm sections, and then observed under a stereomicroscope (Leica, Germany).
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  Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to measure the extracellular insoluble Aβ plaque deposits in the whole brain of mice.
  Method: For brain tissue staining.
  1. Wash mouse brain tissues for three times in PBS (5 min).
  2. Stain sliced brain tissues with Thioflavine S (500 μM; 8 min; room temperature) solution dissolved in 50% ethanol.
  3. Subsequently, after incubating the tissues twice in 50% ethanol for 3 min, the tissues are washed with PBS for 5 min.
  4. Brain tissue sections are mounted on glass slides using a TissueFAXS plus (TissueGnostics GmbH, Vienna, Austria) and using a Zeiss Axio Imager Z2 Microscope System (Carl Zeiss, Jena, Germany) for image.
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  Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to evaluate amyloid deposition.
  Method: For brain tissue staining.
  1. Testing sections are immersed in Thioflavine S (1%; 5 min), hydrated in 70% ethanol and washed for 5 min with PBS for twice.
  2. The sections are sealed with a fluorescent mounting media. Pannoramic MIDI is used to scan the images and analyze the data.
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  Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to measure the aggregation of α-syn.
  Method: For brain sections staining.
  1. Stain sections with Thioflavine S solution (10 mg/mL; 5 min).
  2. Wash cells for twice.
  3. The sections are mounted on slides and images are gathered using a fluorescence microscope (Olympus).
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  Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker, it can be used to monitor the accumulation of Aβ.
  Method: For cell staining.
  1. Inoculate cells on a 24-well culture plate and fix cells with 4% paraformaldehyde (15 min; 25℃).
  2. Add 80% ethanol to percolate at -20℃ for 6 min.
  3. Add Thioflavine S (0.1%; 5 min; 25℃) to percolate for incubate cells.
  4. Use the ImageXpress High-content Imaging system for image. Image Pro Plus 6.0 Image analysis software is used to detect the average fluorescence density.