Nalidixic acid, aquinoloneantibiotic, is effective against both gram-positive and gram-negative bacteria. Nalidixic acid acts in a bacteriostatic manner in lower concentrations and is bactericidal in higher concentrations. Nalidixic acid inhibits a subunit ofDNA gyraseandtopoisomeraseIVand reversibly blocks DNA replication in susceptible bacteria^[1].

Nalidixic acid is against a variety of microorganisms, it is against withEscherichia coli,Pasteurella spp.,Klebsiella pneuiiioniae,Aerobacter aeroyenes,Proteus spp.,Salmonella spp.,Shigella spp.andBrucella spp.with MIC values of 5.0-12.5 μg/ml, 0.5-2.5 μg/ml, 0.8-25.0 μg/ml, 1.0-25.0 μg/ml, 1.25-30.0 μg/ml, 8-3.2 μg/ml, and 7.5-10.0 μg/ml, respectively^[1].

The in vivo activity of Nalidixic acid is most pronounced against Gram-negative bacteria, while Gram-positive organisms are generally more resistant. Maximal activity is observed against systemic infections caused byE. coli,A. aerobacter,Proteus mirabilis,Shigella fkxneri, the ED₅₀values are 25 mg/kg, 60 mg/kg, 50 mg/kg, and 62 mg/kg, respectively^[1].
The acute toxicity (LD₅₀) of Nalidixic acid in mice following oral and parenteral administration is: oral, 3300 mg/kg; intravenous, 176 mg/kg, and subcutaneous, 500 mg/kg^[1].

232.24

Solid

C₁₂H₁₂N₂O₃

389-08-2

萘啶酸

• Antibiotics
• Other Antibiotics

Streptomyces sp.

Room temperature in continental US; may vary elsewhere.

DMSO : 6 mg/mL(25.84 mM;Need ultrasonic)

H₂O : 5 mg/mL(21.53 mM;ultrasonic and warming and heat to 80℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 0.5 mg/mL (2.15 mM); Clear solution

  此方案可获得 ≥ 0.5 mg/mL (2.15 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 5.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。