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FTY720 hydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
FTY720 hydrochloride图片
CAS NO:162359-56-0
包装:100mg, 200mg, 500mg
包装与价格:
包装价格(元)
Free Sample (0.5-1 mg)电议
100mg电议
200mg电议
500mg电议
1g电议

产品名称
Fingolimod
产品介绍

生物活性

Fingolimod (FTY720) HCl是一种1-磷酸-神经鞘氨醇S1P受体拮抗剂,IC50为0.33 nM。S1P 作用于K562细胞,抑制NK细胞裂解。多种浓度 FTY720可逆转S1P的抑制作用,IC50为173 nM。此外, 10 nM FTY720 单独温育时,对共刺激分子的表达没有任何影响。


化学数据

分子量343.9
分子式C19H33NO2.HCl
CAS号162359-56-0
纯度99.46%
溶解性(25°C)DMSO 60 mg/mL
储存和运输条件固体粉末: -20°C 冷藏长期储存
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系MCF-7 cells
方法In Vitro Proliferation Assay Cells were seeded onto 96- well plates at the densities of 3000 cells/well. After 24 h of pre-cultivation, appropriate concentrations of the samples were added and the cells were cultured for 2 d. The relative growth ratio was determined by WST-1 assay using a cell counting kit (Dojindo Laboratories, Kumamoto, Japan) according to the manufacturer’s instructions. The IC50 value was determined from the dose response curve. At least three independent experiments were performed. The proliferation assay that tested the effects of DMS was performed using identical methods. Compounds with or without DMS were added to the wells and the plates were incubated for 78 h at 37 °C in a humidified atmosphere containing 5% CO2 before the WST-1 assay was conducted.
浓度0~100μM
处理时间2 days

动物实验
动物模型Six-weekold female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice with Mino cells
配制not mentioned
剂量10 mg/kg daily for 10 days
给药处理intraperitoneal injections

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM2.9078 mL14.5391 mL29.0782 mL
5 mM0.5816 mL2.9078 mL5.8156 mL
10 mM0.2908 mL1.4539 mL2.9078 mL