Balicatib

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Balicatib

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	354813-19-7
包装:	1mg, 2mg, 5mg, 10mg

包装与价格：

产品名称

AAE-581

产品介绍

Balicatib是一种有效的、选择性的组织蛋白酶K抑制剂;在基于细胞的酶占用试验中比对组织蛋白酶B, L和S的作用强10-100倍。

细胞实验
细胞系	Hep G2 and SMMC-7721 cells
方法	Cell viability assay. The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to measure drug sensitivity. Briefly, Hep G2 and SMMC-7721 cells were seeded into 96-well plates (Corning, USA) at a density of 5 × 10³cells per well, incubated overnight, and then treated with various concentrations (0, 10, 20, 40, and 80 μmol/L) of baicalin for 24, 48, and 72 h. Thereafter, 20 μl of MTT solution (5 mg/mL; Sigma-Aldrich, St. Louis, MO, USA) was added to each well, and the plates were incubated at 37°C for 4 h. The formed formazan crystals were dissolved in 100 μl of DMSO after removal of the supernatant. The optical density (OD) was recorded at 490 nm using a microplate reader (Bio-Tek, Winooski, VT, USA). The percentage of cell viability was calculated as: (OD of baicalin-treated group/OD of control group) × 100%.
浓度	0, 10, 20, 40, and 80 μmol/L
处理时间	24, 48 and 72 h

动物实验
动物模型	Six-week old athymic nude mice bearing Hep G2 cells xenograft model
配制	10% DMSO and 90% propylene glycol
剂量	50 mg/kg and 100 mg/kg daily for 3 weeks
给药处理	i.p.

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.4299 mL	12.1495 mL	24.299 mL
5 mM	0.486 mL	2.4299 mL	4.8598 mL
10 mM	0.243 mL	1.2149 mL	2.4299 mL