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NHC-triphosphate tetrasodium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
NHC-triphosphate tetrasodium图片
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议

产品介绍
NHC-triphosphate tetrasodium是 NHC (HY-125033) 的活性胞内磷酸盐代谢物 (intracellular metabolite),以三磷酸盐的形式存在。NHC-triphosphate tetrasodium是病毒聚合酶 (viral polymerase) 的弱底物替代物,会被并入 HCV 复制子 RNA 中。
生物活性

NHC-triphosphate tetrasodium is an active phosphorylatedintracellular metaboliteof β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form[1]. NHC-triphosphate tetrasodium is a weak alternative substrate for theviral polymeraseand can be incorporated intoHCVreplicon RNA[1][2].

体外研究
(In Vitro)

In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, andNHC-TPreaches up to 71.12 pM after 8 hours[1].
NHC-triphosphate (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate (HY-135867) remains the most abundant metabolite[2].
NHC-triphosphate (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.

分子量

587.08

性状

Solid

Formula

C9H12N3Na4O15P3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

H2O : 100 mg/mL(170.33 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.7033 mL8.5167 mL17.0335 mL
5 mM0.3407 mL1.7033 mL3.4067 mL
10 mM0.1703 mL0.8517 mL1.7033 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。