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Amantadine sulfate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Amantadine sulfate图片
CAS NO:31377-23-8
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
1-Adamantanamine Sulfate
1-Aminoadamantane sulfate
产品介绍
Amantadine (1-Adamantanamine) sulfate 是一种口服有效的抗甲型流感 (influenza A) 病毒的抗病毒剂。Amantadine sulfate 对NMDAM2等多种离子通道均有抑制作用。Amantadine sulfate 还具有抗正痘病毒 (orthopoxvirus) 和抗癌活性。Amantadine sulfate 可用于帕金森病,术后认知功能障碍 (POCD) 和 COVID-19 的研究。
生物活性

Amantadine (1-Adamantanamine) sulfate is an orally avtive and potent antiviral agent with activity againstinfluenza Aviruses. Amantadine sulfate inhibits several ion channels such asNMDAandM2, and also inhibits Coronavirus ion channels. Amantadine sulfate also has anti-orthopoxvirusand anticancer activity. Amantadine sulfate can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research[1][2][3][4][5][6].

IC50& Target

CDK2

 

Bcl-2

 

Bax

 

体外研究
(In Vitro)

Amantadine sulfate (0-500 μM, 26 h) inhibits SARS-CoV-2 replication, with IC50concentrations between 83 and 119 μM[4].
Amantadine sulfate (0-100 μg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC-7721 cells[6].
Amantadine sulfate (0-75 μg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis[6].
Amantadine sulfate (0-75 μg/mL, 48 h) reduces the levels of the cell cycle-related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels[6].

Cell Viability Assay[4]

Cell Line:Vero E6 cells
Concentration:500 μM, 100 μM, 20 μM, 4 μM, and 8 nM
Incubation Time:26 h
Result:Caused a concentration-dependent reduction (IC50=83 μM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 μM. Caused a concentration-dependent reduction (IC50=119 μM) of viral nucleic acids in the cytosol 26 h after infection.

Cell Proliferation Assay[6]

Cell Line:Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells)
Concentration:0, 1, 2, 5, 10, 25, 50 and 100 μg/mL
Incubation Time:24, 48 and 72 h
Result:Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells.

Cell Cycle Analysis[6]

Cell Line:HepG2 and SMMC-7721 cells
Concentration:0, 10, 25, 50 and 75 μg/mL
Incubation Time:48 h
Result:Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase.

Apoptosis Analysis[6]

Cell Line:HepG2 and SMMC-7721 cells
Concentration:0, 10, 25, 50 and 75 μg/mL
Incubation Time:48 h
Result:Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner.

Western Blot Analysis[6]

Cell Line:HepG2 and SMMC-7721 cells
Concentration:0, 10, 25, 50 and 75 μg/mL
Incubation Time:48 h
Result:Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells.

RT-PCR[6]

Cell Line:HepG2 and SMMC-7721 cells
Concentration:0, 10, 25, 50 and 75 μg/mL
Incubation Time:48 h
Result:Revealed an increase in Bax and decrease in Bcl-2 genes.
体内研究
(In Vivo)

Amantadine sulfate (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment[5].

Animal Model:Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group)[5]
Dosage:25 mg/kg
Administration:IP, once daily for 3 days (the first dose at 15 min before surgery)
Result:Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.
Clinical Trial
分子量

200.18

Formula

C10H17N.1/2H2O4S

CAS 号

31377-23-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.