您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Berberine chloride hydrate
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Berberine chloride hydrate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Berberine chloride hydrate图片
CAS NO:68030-18-2
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
100mg电议
1 g电议
5 g电议
10 g电议
50 g电议

产品名称
盐酸黄连素水合物
Natural Yellow 18 chloride hydrate
产品介绍
Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) 是一种生物碱,常用作抗生素。Berberine chloride hydrate 诱导活性氧 (ROS) 生成并抑制 DNA 拓扑异构酶 (topoisomerase)。抗肿瘤特性。
生物活性

Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) is an alkaloid that acts as anantibiotic. Berberine chloride hydrate inducesreactive oxygen species(ROS) generation and inhibitsDNAtopoisomerase. Antineoplastic properties[1].

IC50& Target

ROS[1]
DNA topoisomerase[1]

体外研究
(In Vitro)

Berberine chloride hydrate ( Natural Yellow 18 chloride hydrate; 1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine chloride hydrate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine chloride hydrate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine chloride hydrate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

Cell Proliferation Assay[1]

Cell Line:Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
Concentration:1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:72 hours
Result:Inhibited the proliferation of four cell lines. The IC50ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

Cell Proliferation Assay[1]

Cell Line:Colorectal carcinoma cell lines LoVo
Concentration:1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
Incubation Time:24, 48, 72 hours
Result:Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

Cell Cycle Analysis[1]

Cell Line:LoVo cells
Concentration:0, 10, 20, 40, or 80 μM
Incubation Time:24 hours
Result:Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

Western Blot Analysis[1]

Cell Line:LoVo cells
Concentration:10, 20, 40, or 80 μM
Incubation Time:24 hours
Result:Suppressed cyclin B1, cdc2 and cdc25c protein expression.
体内研究
(In Vivo)

Berberine chloride hydrate (Natural Yellow 18 chloride hydrate; 10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine chloride hydrate at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

Animal Model:5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
Dosage:10, 30, or 50 mg/kg/day
Administration:Gastrointestinal gavage; for 10 consecutive days
Result:Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.
Clinical Trial
分子量

389.83

性状

Solid

Formula

C20H20ClNO5

CAS 号

68030-18-2

中文名称

盐酸小檗碱水合物;盐酸黄连素水合物

结构分类
  • Alkaloids
  • Isoquinoline Alkaloids
来源
  • Plants
  • Ranunculaceae
  • Coptis chinensisFranch.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : ≥ 3.9 mg/mL(10.00 mM)

H2O : 1 mg/mL(2.57 mM;ultrasonic and warming and heat to 60℃)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.5652 mL12.8261 mL25.6522 mL
5 mM0.5130 mL2.5652 mL5.1304 mL
10 mM0.2565 mL1.2826 mL2.5652 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。