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Lobetyolin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Lobetyolin图片
包装:10mg
市场价:1710元

产品介绍
Lobetyolin 是一种生物活性化合物,来源于党参。

Cell experiment:

After 24 hours of serum deprivation, cells are pretreated with Lobetyolin, lobetyol or methyl linoleate (1, 10, and 100 μM), for 30 minutes and then treated with phorbol 12-myristate 13-acetate (PMA; 10 ng/mL) for 24 hours in serum-free RPMI 1640. After 24 hours, the spent media are collected to measure the secretion of MUC5AC protein and cells are lysed with buffer solution containing 20 mM Tris, 0.5% NP-40, 250 mM NaCl, 3 mM EDTA, 3 mM EGTA, and protease inhibitor cocktail and collected to measure the production of MUC5AC protein (in 24-well culture plate)[1].

产品描述

Lobetyolin is derived from Codonopsis pilosula and has antioxidative effect.

Lobetyolin is derived from Codonopsis pilosula and has antioxidative effect. MUC5AC gene expression induced by phorbol 12-myristate 13-acetate (PMA) from NCI-H292 cells is inhibited by pretreatment with Lobetyolin, lobetyol or methyl linoleate. Lobetyolin does not affect PMA-induced MUC5AC production from NCI-H292 cells. The amounts of mucin in the cells of Lobetyolin-treated cultures are 100±25%, 487±33%, 524±38%, 411±24%, and 402±45% for control, 10 ng/mL of PMA alone, PMA plus Lobetyolin 1 μM, PMA plus Lobetyolin 10 μM, and PMA plus Lobetyolin 100 μM, respectively[1].

References:
[1]. Yoon YP, et al. Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells. Tuberc Respir Dis (Seoul). 2014 Nov;77(5):203-8.