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Batimastat(BB94)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Batimastat(BB94)图片
CAS NO:130370-60-4
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)477.64
FormulaC23H31N3O4S2
CAS No.130370-60-4
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 96 mg/mL (201.0 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In vivo)30% propylene glycol, 5% Tween 80, 65% D5W: 30 mg/mL
SynonymsBB94; Batimastat; BB 94; BB-94.

IUPAC/Chemical Name: (2S,3R)-N-Hydroxy-N'-[(2S)-1-methylamino-1-oxo-3-phenylpropan-2-yl]-3-(2-methylpropyl)-2-(thiophen-2-ylsulfanylmethyl)butanediamide.

InChi Key: XFILPEOLDIKJHX-QYZOEREBSA-N

InChi Code: InChI=1S/C23H31N3O4S2/c1-15(2)12-17(18(22(28)26-30)14-32-20-10-7-11-31-20)21(27)25-19(23(29)24-3)13-16-8-5-4-6-9-16/h4-11,15,17-19,30H,12-14H2,1-3H3,(H,24,29)(H,25,27)(H,26,28)/t17-,18+,19+/m1/s1

SMILES Code: O=C(NO)[C@@H](CSC1=CC=CS1)[C@@H](CC(C)C)C(N[C@@H](CC2=CC=CC=C2)C(NC)=O)=O

实验参考方法
In Vitro

In vitro activity: Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site.


Kinase Assay: Batimastat is a potent broad spectrum MMP inhibitor with IC50 of 3, 4, 4, 6, and 20 nM for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3, respectively.


Cell Assay: In vitro proliferation in the presence of batimastatwas assessed by the MTT absorbance assay. The effect of batimastat on the in vitro growth of C170HM2 and AP5LV in both serum-free and scrum-containing culture media was determined three times. Batimastat had no significant effect on the growth of either cell line.

In VivoBatimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%). Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures.
Animal modelNude mice
Formulation & DosageDissolved in PBS containing 0.01% Tween 2O; 30 mg/kg; i.p. injection
References

Int J Cancer. 1994 Aug 1;58(3):460-4; Cancer Res. 1994 Sep 1;54(17):4726-8; J Natl Cancer Inst. 1995 Feb 15;87(4):293-8.

生物活性