Angiotensin II human (Angiotensin II) TFA 是一种血管收缩剂，是肾素/血管紧张素系统的主要生物活性肽。Angiotensin II human TFA 在调节人类血压中起着核心作用，主要通过血管紧张素 II 与 G 蛋白偶联受体 (GPCRs)、血管紧张素II 1型受体 (AT1R) 和血管紧张素II 2型受体 (AT2R) 之间的相互作用来介导。Angiotensin II human TFA 刺激交感神经刺激，增加醛固酮生物合成和肾脏活动。Angiotensin II human TFA 诱导血管平滑肌细胞生长，增加成纤维细胞中 I 型和 III 型胶原的合成，导致血管壁和心肌增厚，并导致纤维化。Angiotensin II human TFA 也诱导细胞凋亡。Angiotensin II human TFA 通过 LOX-1 依赖的氧化还原敏感途径诱导内皮细胞毛细血管形成。
| 生物活性 | Angiotensin II human (Angiotensin II) TFA is a vasoconstrictor and a major bioactive peptide of the renin/angiotensin system. Angiotensin II human TFA plays a central role in regulating human blood pressure, which is mainly mediated by interactions between Angiotensin II and theG-protein-coupled receptors (GPCRs)Angiotensin II type 1 receptor (AT1R) and Angiotensin II type 2 receptor (AT2R). Angiotensin II human TFA stimulates sympathetic nervous stimulation, increases aldosterone biosynthesis and renal actions. Angiotensin II human TFA induces growth of vascular smooth muscle cells, increases collagen type I and III synthesis in fibroblasts, leading to thickening of the vascular wall and myocardium, and fibrosis. Angiotensin II human TFA also inducesapoptosis. Angiotensin II human TFA induces capillary formation from endothelial cells via theLOX-1dependent redox-sensitive pathway[1][2][3][4]. |
体外研究
(In Vitro) | Most of the known actions of Angiotensin II (Ang II) human are mediated by AT1 receptors, the AT2 receptor contributes to the regulation of blood pressure and renal function[1].
Angiotensin II human raises blood pressure (BP) by a number of actions, the most important ones being vasoconstriction, sympathetic nervous stimulation, increased aldosterone biosynthesis and renal actions. Other Angiotensin II human actions include induction of growth, cell migration, and mitosis of vascular smooth muscle cells, increased synthesis of collagen type I and III in fibroblasts, leading to thickening of the vascular wall and myocardium, and fibrosis. These actions are mediated by type 1 Ang II receptors (AT1)[2].
Angiotensin II (1 nM) TFA induces the expression of LOX-1 and VEGF and enhances capillary formation from human coronary endothelial cells in Matrigel assay. Angiotensin II-mediated expression of LOX-1 and VEGF, capillary formation, intracellular reactive oxygen species generation, and phosphorylation of p38 as well as p44/42 mitogen-activated protein kinases, are suppressed by anti-LOX-1 antibody, nicotinamide-adenine dinucleotide phosphate oxidase inhibitor apocynin and the Ang II type 1 receptor blocker Losartan, but not by the Ang II type 2 receptor blocker PD123319[3].
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体内研究
(In Vivo) | Angiotensin II human (5 mL of 1 nM; intraperitoneal injection; 200-250 g Sprague-Dawley rats) TFA induces a significant neutrophil recruitment that was maximal at 4 hours and had resolved by 24 hours[4].
To distinguish the AT1receptor population that is critical for the pathogenesis of hypertension, osmotic minipumps are implanted s.c. into each animal to infuse Angiotensin II human (1000 ng/kg/min) acetate continuously for 4 weeks. Angiotensin II human acetate causes hypertension by activating AT1receptors in the kidney promoting sodium reabsorption[5].
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Protect from light, stored under nitrogen | Powder | -80°C | 2 years | | -20°C | 1 year |
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
| 溶解性数据 | In Vitro: H2O : 10 mg/mL(8.62 mM;Need ultrasonic) 配制储备液 | 1 mM | 0.8619 mL | 4.3096 mL | 8.6192 mL | | 5 mM | 0.1724 mL | 0.8619 mL | 1.7238 mL | | 10 mM | --- | --- | --- |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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