6,8- diprenylorobol 是一种烯酰化异黄酮，是一种可以从Cudrania tricuspidata叶片中分离出来的天然产物。6,8- diprenylorobol 具有抗增殖作用，并通过激活 p53 和产生 ROS 诱导细胞凋亡 (apoptosis)。
| 生物活性 | 6,8-Diprenylorobol, a prenylated isoflavone, is a nature product that could be isolated from the leaves ofCudrania tricuspidata. 6,8-Diprenylorobol antiproliferative effect and inducesapoptosisthrough activation of p53 and generation of ROS[1][2]. |
体外研究
(In Vitro) | 6,8-Diprenylorobol (20-60 μM; 24-72 h) 抑制 LoVo 和 HCT15 细胞增殖[1]。
6,8-Diprenylorobol (20-60 μM;24 h) 诱导 LoVo 和 HCT15 细胞凋亡，激活 p53 并调控 MAPKs[1]。
6,8-Diprenylorobol (20-60 μM; 1 h) 增加 LoVo 和 HCT15 细胞的 ROS 水平[1]。
Cell Viability Assay[1] | Cell Line: | LoVo and HCT15 cells | | Concentration: | 20, 40, and 60 μM | | Incubation Time: | 24, 48, and 72 hours | | Result: | Inhibited the growth of LoVo and HCT15 cells in a dose- and dose-dependent manner. |
Apoptosis Analysis[1] | Cell Line: | LoVo and HCT15 cells | | Concentration: | 20, 40, and 60 μM | | Incubation Time: | 24 hours | | Result: | Induced apoptosis of LoVo and HCT15 cells in a dose- and time-dependent manner. |
Western Blot Analysis[1] | Cell Line: | LoVo and HCT15 cells | | Concentration: | 20, 40, and 60 μM | | Incubation Time: | 24, 48, and 72 hours | | Result: | Decreased the expression of PARP and increased the expression of cleaved PARP.
Up-regulated Bax and Bim expressions and downregulated Bcl-2 expression.
Up-regulated cleaved caspase-3, -7, -8, and -9 expressions, and down-regulated procaspase-3, -7, -8, and -9 expressions.
Decreased the expression of phosphorylated Akt, ERK, JNK, and p38 and increased the expression of FOXO3, p53, p27, and p21.
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| 来源 | - Plants
- Moraceae
- Maclura tricuspidataCarriere
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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