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Bcl-2-IN-8
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Bcl-2-IN-8图片
包装与价格：
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
Bcl-2-IN-8 是一种有效的抗癌剂。Bcl-2-IN-8 对药物敏感和耐药的癌细胞均显示抗增殖活性。Bcl-2-IN-8 诱导细胞凋亡 (apoptosis) 和细胞周期停滞在 G1 期。Bcl-2-IN-8 以剂量依赖性方式抑制细胞迁移。Bcl-2-IN-8 具有三阴乳腺癌的研究潜力。
生物活性

Bcl-2-IN-8 is a potent anticancer agent. Bcl-2-IN-8 shows anti-proliferative activity against both drug-sensitive and drug-resistantcancercells. Bcl-2-IN-8 induceapoptosisand cell cycle arrest at G1 phase. Bcl-2-IN-8 inhibits cell migration in a dose-dependent manner. Bcl-2-IN-8 has the potential for the research of triple negrative breastcancer[1].

IC50& Target[1]

Bax

 

Bcl-2

 

体外研究
(In Vitro)

Bcl-2-IN-8 (compound 4m) (48 h) shows selective cytotoxicity against MDA-MB-231, MCF-7/ADR, MCF-10A cells with IC50s of 0.51, 0.38, 3.56 μM, respectively[1].
Bcl-2-IN-8 (0-8 μM; 24, 48, 72 h) inhibits cell proliferation in a dose-time dependently[1].
Bcl-2-IN-8 (0-2 μM; 24 h) induces cell cycle arrest at G1 phase in MDA-MB-231 cells[1].
Bcl-2-IN-8 (0-2 μM; 24 h) induces apoptosis in MDA-MB-231 cells[1].
Bcl-2-IN-8 (0-3 μM; 24 h) up-regulates the expression of Bax, cytochrome c and down-regulates Bcl-2, P53 protein in a concentration dependently in MDA-MB-231 cells[1].
Bcl-2-IN-8 (0-3 μM; 24 h) decreases the ΔΨm and induces ROS (reactive oxygen species) generation by activating the p38MAPK-Akt signaling pathway in MDA-MB-231 cells[1].
Bcl-2-IN-8 (0-2 μM; 24 h) increases intracellular Ca2+concentration from 9.9 treated with DMSO to 37.4 treated at 2 μM in MDA-MB-231 cells[1].
Bcl-2-IN-8 (0-3 μM; 24 h) significantly inhibits migration in a dose-dependent manner in MDA-MB-231 cells[1].
Bcl-2-IN-8 (0-3 μM; 24 h) increases the expression levels of Smad 7 but decreases the expression levels of p-Smad 3 in MDA-MB-231 cells[1].

Cell Cytotoxicity Assay[1].

Cell Line:MB-231, MCF-7, MCF-7/adriamycin (adriamycin-resistant MCF-7 cell line) cells
Concentration:
Incubation Time:48 h
Result:Showed potent inhibitory activities on MDA-MB-231, MCF-7, MCF-7/ADR (adriamycin) cells with IC50s of 0.51, 2.01, 0.38 μM, respectively.

Cell Proliferation Assay[1].

Cell Line:MDA-MB-231 cells
Concentration:0-8 μM
Incubation Time:24, 48, 72 h
Result:Inhibited cell proliferation in a dose-time dependently.

Cell Cycle Analysis[1].

Cell Line:MDA-MB-231 cells
Concentration:0.5, 1, 2 μM
Incubation Time:24 h
Result:Arrested the cell-cycle at the G1 phase and the percentage of cells in the G1 phase from 54.8 to 74.6%.

Western Blot Analysis[1].

Cell Line:MDA-MB-231 cells
Concentration:0.5, 1, 2, 3 μM
Incubation Time:24 h
Result:Decreased the expression of cyclin D1, CDK4 and CDK6 protein, but did not influence the expression of c-myc proteins.

Apoptosis Analysis[1].

Cell Line:MDA-MB-231 cells
Concentration:0, 0.5, 1, 2 μM
Incubation Time:24 h
Result:The total percentage of early and late apoptosis increased to 10.2%, 17.6%, 56.4% with the concentration of 0.5 μM, 1 μM, 2 μM.
体内研究
(In Vivo)

Bcl-2-IN-8 shows metabolic stability with CL50value of 14.1 mL/min/mg and remaining (T=100 min) of 18.5% in rats[1].

分子量

572.73

Formula

C36H44O6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.