Cimiside E (25-Anhydrocimigenol xyloside) 是一种三萜木糖苷，对胃癌细胞有凋亡作用，IC50为 14.58 μM。Cimiside E 诱导细胞周期阻滞，并通过诱导caspase级联的外部和内在途径介导细胞凋亡。
| 生物活性 | Cimiside E (25-Anhydrocimigenol xyloside) is a triterpene xyloside, Cimiside E possesses apoptotic action on gastriccancercells, with an IC50value of 14.58 μM. Cimiside E induces cell cycle arrest at G2/M phase, and mediatesapoptosisthrough the induction of thecaspasecascade for both the extrinsic and intrinsic pathways[1][2]. |
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体外研究
(In Vitro) | Cimiside E (30-90 μM; 24 h) 阻滞细胞周期，诱导 ASG 细胞凋亡[1]。
Cimiside E (30-90 μM; 12-48 h) 对 AGS 细胞有较强的细胞毒性，并表现出抗增殖活性[1]。
Cimiside E (15-60 μM; 6-24 h) 诱导 ASG细胞中 DNA 片段化，(30-60 μM; 1-6 h) 并在 3 h 时激活 FasL 的表达，在 1 h 激活 Fas[1]。
Cimiside E (30-90 μM; 3-24 h) 介导 caspase 级联，增加 Bax/Bcl-2 比值，降低突变型 p53 和 procaspase 3 蛋白水平[1]。
Western Blot Analysis[1] | Cell Line: | Cimiside E. AGS cells | | Concentration: | 30 μM, 60 μM, and 90 μM | | Incubation Time: | 3 h, 6 h, 12 h, and 24 h | | Result: | Increased the ratio of Bax/Bcl-2 expression from 60 μM.
Decreased mutant type (mt) p53 levle from 12 h at 30 μM.
Suppressed the protein level of procaspase 3 in a dose-dependent manner from 30 μM. |
Cell Proliferation Assay[1] | Cell Line: | Cimiside E. AGS cells | | Concentration: | 30 μM, 60 μM, and 90 μM | | Incubation Time: | 12 h, 24 h, and 48 h | | Result: | Inhibited ASG cells proliferation with IC50s of 28.7, 14.6 and 8.1 μM, respectively, for 30 μM, 60 μM, and 90 μM treatment. |
Cell Cycle Analysis[1] | Cell Line: | Cimiside E. AGS cells | | Concentration: | 30 μM, 60 μM, and 90 μM | | Incubation Time: | 3 h, 6 h, and 24 h | | Result: | Induced cell cycle arrest at S phase in a low concentration (30 μM), but arrested cell cycle at G2/M phase in higher concentration (60 μM and 90 μM). |
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| 来源 | - Plants
- Ranunculaceae
- Cimicifuga racemosa
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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