FOXO4-DRI 是一种细胞渗透性肽拮抗剂，可阻断FOXO4和p53的相互作用。FOXO4-DRI 是一种抗衰老多肽，可诱导衰老细胞凋亡 (apoptosis)。
| 生物活性 | FOXO4-DRI is a cell-permeable peptide antagonist that blocks the interaction ofFOXO4andp53. FOXO4-DRI is a senolytic peptide that inducesapoptosisof senescent cells[1]. |
体外研究
(In Vitro) | FOXO4-DRI (25 mM; 3 days) 引起活性 p53 的核排斥，诱导衰老 TM3 Leydig 细胞凋亡[1]。
FOXO4-DRI (25 μM; 5 days) 显著降低 PDL9 细胞的衰老水平[2]。
Cell Viability Assay[1] | Cell Line: | Senescent Leydig cells | | Concentration: | 25 mM | | Incubation Time: | 3 days | | Result: | Reduced the viability of senescent as compared to normal TM3 Leydig cells. |
Apoptosis Analysis[1] | Cell Line: | Senescent Leydig cells | | Concentration: | 25 mM | | Incubation Time: | 3 days | | Result: | The apoptosis rate increased from 10% to 27%. |
Western Blot Analysis[2] | Cell Line: | PDL9 cells | | Concentration: | 25 μM | | Incubation Time: | 5 days | | Result: | Decreased the protein levels of representative senescent markers, including p16, p21, and p53. |
RT-PCR[2] | Cell Line: | PDL9 cells | | Concentration: | 25 μM | | Incubation Time: | 5 days | | Result: | EnhancedSOX9expression, and reducedMMP12andMMP13expression. |
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体内研究
(In Vivo) | FOXO4-DRI (5 mg/kg; i.p.; every other day for three administrations) 缓解自然衰老小鼠睾丸激素分泌不足，改善睾丸微环境[1]。
| Animal Model: | Naturally aged male C57BL/6 mice (20-24 months old)[1] | | Dosage: | 5 mg/kg | | Administration: | Intraperitoneal injection, every other day for three administrations | | Result: | Increased serum testosterone levels. Increased levels of both 3β-HSD and CYP11A1. Decreased interstitial SA-β-gal activity and lowered levels of senescence-associated proteins p53, p21, and p16. Decreased the levels of IL-1β, IL-6 and TGF-β. |
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| 分子量 | |
| Formula | |
| CAS 号 | |
| Sequence | D-(Leu-Thr-Leu-Arg-Lys-Glu-Pro-Ala-Ser-Glu-Ile-Ala-Gln-Ser-Ile-Leu-Glu-Ala-Tyr-Ser-Gln-Asn-Gly-Trp-Ala-Asn-Arg-Arg-Ser-Gly-Gly-Lys-Arg-Pro-Pro-Pro-Arg-Arg-Arg-Gln-Arg-Arg-Lys-Lys-Arg-Gly) |
| Sequence Shortening | D-(LTLRKEPASEIAQSILEAYSQNGWANRRSGGKRPPPRRRQRRKKRG) |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Solvent & Solubility | In Vitro: H2O Peptide Solubility and Storage Guidelines: 1. Calculate the length of the peptide. 2. Calculate the overall charge of the entire peptide according to the following table: | | Contents | Assign value | | Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 | | Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 | | Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution: | Overall charge of peptide | Details | | Negative (<0) | 1. Try to dissolve the peptide in water first.
2. If water fails, add NH4OH (<50 μL).
3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. | | Positive (>0) | 1. Try to dissolve the peptide in water first.
2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. | | Zero (=0) | 1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
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