C16-Ceramide 是一种天然小分子，通过直接选择性结合激活p53。
| 生物活性 | C16-Ceramide is a natural small molecule activatingp53through the direct and selective binding[1]. |
| IC50& Target | |
体外研究
(In Vitro) | C16-Ceramide interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide in the cell[1].
C16-Ceramide (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner[2].
C16-Ceramide (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells[2].
C16-ceramide (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-ceramide down-regulates Mdm2 expression via Btf[2].
Cell Viability Assay[2] | Cell Line: | HCT116 cells | | Concentration: | 2.5, 5, 10, 12, 20, 50 μM | | Incubation Time: | 0-48 h | | Result: | Strongly decreased cell viability in a time- and concentration-dependent manner. |
Western Blot Analysis[2] | Cell Line: | HCT116 cells | | Concentration: | 12 μM | | Incubation Time: | 1, 3 and 6 h | | Result: | Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion also
partially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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