GN25 是一种特异性的p53-Snail binding抑制剂，具有抗肿瘤作用。
| 生物活性 | GN25 is a specificp53-Snail bindinginhibitor with antitumor effects[1]. |
体外研究
(In Vitro) | GN25 (10 and 20 μM; 24 h) inhibits cell viability of K-Ras-mutated MEF cells[1].
GN25 (5 μM; 4 h) activates p53 in a K-Ras-dependent manner[1].
GN25 (1-10 μM; 1-6 h) induces p53 and p21 in a dose- and time- dependent manner in K-Ras-mutated A549 and HCT116 cell lines[1].
GN25 activates wild-type p53 in p53WT/MTcells[1].
GN25 (0.25 μM) recovers the masking effect of p53 on CK1/GSK3b-mediated Snail phosphorylation[1].
Cell Viability Assay[1] | Cell Line: | HCT116, A459 and MKN45 cell lines | | Concentration: | 10 μM | | Incubation Time: | 24 h | | Result: | Obviously suppressed the cell viability of HCT116 and A459 cell lines. |
Cell Proliferation Assay[1] | Cell Line: | K-Ras-transformed and N-Ras/Myc-transformed MEF cells | | Concentration: | 10 μM and 20 μM | | Incubation Time: | 24 h | | Result: | Inhibited the viability of K-Ras-transformed but not N-Ras/Myc-transformed MEF cells. |
Western Blot Analysis[1] | Cell Line: | K-Ras-MEF, N-Ras/Myc-MEF and Nontransformed MEF cells | | Concentration: | 5 μM | | Incubation Time: | 4 h | | Result: | Only induced p53 and p21 in K-Ras-MEF cells. |
Western Blot Analysis[1] | Cell Line: | K-Ras-mutated A549, HCT116 cell lines and MKN45 | | Concentration: | 1, 5, and 10 μM | | Incubation Time: | 1, 3, and 6 h | | Result: | Induced p53 and p21 in a dose- and time- dependent manner in A549 and HCT116 cells. |
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体内研究
(In Vivo) | GN25 (10 and 20 mg/kg; i.p. once a week for 10 weeks) blocks the tumor progression and induces tumor regression in mice[1].
| Animal Model: | Athymic mice with A549 xenografts[1] | | Dosage: | 10 and 20 mg/kg | | Administration: | Intraperitoneal injection; once a week for 10 weeks | | Result: | Obviously regressed tumors in mice. Showed no significant toxicity in liver, pancreas and kidney. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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