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MESG(7-methyl-6-Thioguanosine)
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
MESG(7-methyl-6-Thioguanosine)图片
CAS NO:55727-10-1
包装与价格：
包装价格(元)
5mg电议
10mg电议

产品介绍
MESG (7-methyl-6-Thioguanosine) (MESG) 是一种发色底物，可用于无机磷酸盐的定量。
Cas No.55727-10-1
别名MESG
化学名2-amino-6,9-dihydro-7-methyl-9-β-D-ribofuranosyl-6-thioxo-1H-purinium, inner salt
Canonical SMILESS=C1NC(N)=NC2=C1[N+](C)=CN2[C@H]3[C@@H]([C@@H]([C@@H](CO)O3)O)[O-]
分子式C11H15N5O4S
分子量313.3
溶解度DMF: 50 mg/ml,DMSO: 30 mg/ml,PBS (pH 7.2): 10 mg/ml
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

7-methyl-6-Thioguanosine is a chromophoric substrate that is used to quantify inorganic phosphate (Pi). In the presence of purine nucleoside phosphorylase, it reacts with Pi to produce 7-methyl-6-thioguanine and ribose 1-phosphate, with the increase in 7-methyl-6-thioguanine followed by continuous spectrophotometry at an absorbance of 355-360 nm.1 7-methyl-6-Thioguanosine is also used to measure changes in Pi levels in coupled enzyme systems, including assays of the activities of protein phosphatases, phosphorylase kinases, GTPases, and ATPases.[1],[2],[3],[4]

Reference:
[1]. Webb, M.R. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proceedings of the National Academy of Sciences of the United States of America 89(11), 4884-4887 (1992).
[2]. Cheng, Q., Wang, Z.X., and Killilea, S.D. A continuous spectrophotometric assay for protein phosphatases. Analytical Biochemistry 310(2), 156-162 (2002).
[3]. Wang, Z.X., Cheng, Q., and Killilea, S.D. A continuous spectrophotometric assay for phosphorylase kinase. Analytical Biochemistry 230(1), 55-61 (1995).
[4]. Tan, Y.C., Wu, W.N., Zheng, Y., et al. Characterization of the interactions between the small GTPase RhoA and its guanine nucleotide exchange factors. Analytical Biochemistry 310(2), 156-162 (2014).