Hsp90-IN-15 是一种Hsp90抑制剂，显示出抗癌活性。Hsp90-IN-15 诱导细胞凋亡 (apoptosis)，阻滞 S 期细胞周期，并降低 Hela 细胞中 Hsp90 的表达水平。
| 生物活性 | Hsp90-IN-15 is anHsp90inhibitor with anticancer activity. Hsp90-IN-15 induces cellapoptosis, arrests the cell cycle at S phase and decreases the expression level ofHsp90in Hela cell[1]. |
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体外研究
(In Vitro) | Hsp90-IN-15 (compound 22g) againsts HeLa, HepG2, and MDA-MB-23 cells with IC50s of 19.6 μM, 16.1 μM, and 22.1 μM, respectively[1].
Hsp90-IN-15 binds with Hsp90N, and the △Tm value is 10.92 ℃[1].
Hsp90-IN-15 (0-20 μM; 48 h) arrests cell cycle at S phase in a dose-dependent manner[1].
Hsp90-IN-15 (0-20 μM; 48 h) exhibits an accumulation of early and late apoptotic cells in a time and dose dependent manner[1].
Caspase-3 is an executioner caspase and modifies apoptosis proteins[1].
Hsp90-IN-15 (0-50 μM; 48 h) increases the expression of Bax and cleaved-caspase 3, as well as downregulates the levels of Bcl-2, pro-caspase 3 and Hsp90[1].
Cell Viability Assay[1] | Cell Line: | HeLa cells | | Concentration: | 0, 5, 10, 20 μM | | Incubation Time: | 48 hours | | Result: | Arrested cell cycle at S phase dose-dependently. |
Apoptosis Analysis[1] | Cell Line: | HeLa cells | | Concentration: | 0, 5, 10, 20 μM | | Incubation Time: | 48 hours | | Result: | Induced apoptosis in HeLa cells. |
Western Blot Analysis[1] | Cell Line: | HeLa cells | | Concentration: | 0, 12.5, 25, 50 μM | | Incubation Time: | 48 hours | | Result: | Decreased the level of Hsp90 and increased the proportion of Bax/Bcl-2 as well. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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