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WF-536
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
WF-536图片
CAS NO:539857-64-2
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
WF-536 是一种 Rho 相关卷曲螺旋蛋白激酶(ROCK)抑制剂,具有口服活性。WF-536 具有肿瘤抗转移活性。WF-536 可用于癌症研究。
生物活性

WF-536 is an orally active inhibitor ofRho-associated coiled-coil-containing protein kinase (ROCK). WF-536 has tumor anti-metastatic activity. WF-536 can be used for the research ofcancer[1].

体外研究
(In Vitro)

WF-536(1、3、10、30、100 μM)抑制 LLC 细胞的侵袭和迁移以及内皮细胞在 Matrigel 上的侵袭、迁移和毛细管样管的形成,在这两种细胞类型下均无细胞毒性或抗增殖作用[1]

Cell Migration Assay[1]

Cell Line:LLC cells, Human umbilical vein endothelial cells
Concentration:1, 3, 10, 30 μM
Incubation Time:6 h (tumor cells), 4 h (endothelial cells)
Result:Significantly inhibited LLC-cell migration, with inhibitory rates of 23% and 44% at 10 and 30 μM, respectively.

Cell Invasion Assay[1]

Cell Line:LLC cells, Human umbilical vein endothelial cells
Concentration:1, 3, 10, 30 μM
Incubation Time:18 h
Result:Showed significant and concentration-dependent inhibition of LLC-cell invasion.

Cell Proliferation Assay[1]

Cell Line:LLC cells, Human umbilical vein endothelial cells
Concentration:1, 30, 100 μM
Incubation Time:24, 48, 72, and 96 h
Result:Showed no significant effect at 1-30 μM on the proliferation up to 96 h incubation, although the proliferation was decreased at 100 μM over 48 h.
体内研究
(In Vivo)

WF-536(口服;0.3-3 mg/kg/天;持续 28 天)抑制小鼠 Lewis 肺癌 (LLC) 转移和 LLC 诱导的血管生成[1]

Animal Model:C57BL/6 mice (male, 6-week old)[1]
Dosage:0.3, 1, 3 mg/kg
Administration:Oral; daily; for 28 days
Result:Significantly reduced the number of pulmonary metastatic colonies of LLC in a dose-dependent manner (0.3- 3 mg/kg).
分子量

277.75

Formula

C14H16ClN3O

CAS 号

539857-64-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.