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Brassinolide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Brassinolide图片
包装与价格:
包装价格(元)
10mM (in 1mL DMSO)电议
5mg电议
10mg电议

产品介绍
芸苔素内酯是调节植物细胞伸长的主要植物生长调节剂。

Cell lines

Human prostate cancer PC-3 cell

Preparation method

The solubility of this compound in DMSO is >24.1mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

10, 20 and 40 μM; 12, 24 and 36 h

Applications

Brassinolide induced a time and concentration-dependent cytotoxicity in PC-3 cells. Brassinolide (10, 20 and 40 μM, 12 h) induced a concentration-dependent increase in the apoptotic rate and marked accumulation in G2/M phase of cell cycle. PC-3 cells treated with brassinolide (20 μM, 24 h) showed characteristic apoptotic alterations: shrinking cellular figure, decreasing cell surface microvilli, cytoplasmic vacuoles, chromatin condensation. PC-3 cells treated with brassinolide (20 μM) for 6, 12 and 18 h showed a time-dependent increase in the activity of caspases-3.

Animal models

Diabetes rats

Dosage form

Oral administration; 200,100,and 50 mg/kg; once every day for 7 days

Application

Oral administration of Brassinolide decreased the levels of blood glucose from 19.71-24.10 mmol/L to 9.89 mmol/L-12.70 mmol/L. The levels of blood glucose displayed significant differences after treatment with different dose of brassinolide. Brassinolide can still reduce the blood glucose levels without toxicity effect even at a lower dose.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

产品描述

IC50: N/A

Brassinolide is a plant growth regulator. Plant growth regulators are naturally produced by plants and are critical for regulating their own growth. Plant growth regulators act via modifying or controlling plant growth processes, such as leave and flower formation, stem elongation, as well as fruit development and ripening.

In vitro: Brassinolide induced the time and concentration-dependent cytotoxicity in PC-3 cells. The mode of such cell death appeared to be apoptosis predominately, as demonstrated by fluorescence, flow-cytometric analyses and transmission electron microscopes. Moreover, Caspase-3 activity was increased after brassinolide treatment obviously. Western blot studies showed brassinolide treatment triggered a time-dependent decrease in the expression of Bcl-2 [1].

In vivo: Brassinolide had the similar function of reducing the blood glucose levels as phenformin, but without the dose-dependent manner. The blood glucose levels showed significant differences after brassinolide treatment with different doses (200, 100, and 50 mg/kg). These results indicated that brassinolide could reduce the blood glucose levels without toxicity [2].

Clinical trial: So far, no clinical study has been reported.

References:
[1] Wu YD,Lou YJ.  Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie.2007 May;62(5):392-5.
[2] CHEN Shi-ping, HE Jia, WANG Qi-jing, WANG Jian-dong, YE Yan-li, XIAN Li-jian.  Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats. Acta Metallurgica Sinica, 2009,V26(03): 21-23