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Nicotinamide Hydrochloride
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Nicotinamide Hydrochloride图片
CAS NO:25334-23-0
包装与价格：
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
Niacinamide Hydrochloride
Nicotinic acid amide Hydrochloride
产品介绍
Nicotinamide Hydrochloride 是维生素 B3 或烟酸的一种形式，可抑制SIRT2的体外活性，其EC50值为 2 μM。 Nicotinamide Hydrochloride 可抑制 90% 的黑色素瘤细胞数量，并增加细胞内 NAD+、ATP、ROS 水平。Nicotinamide Hydrochloride 抑制黑色素瘤小鼠的肿瘤生长并提高生存率，可用于黑色素瘤等皮肤癌相关的研究。
生物活性

Nicotinamide Hydrochloride is a form of vitamin B3 or niacin that inhibitssirtuin2 (SIRT2) activityin vitro, with anEC50of 2 μM. Nicotinamide Hydrochloride inhibits up to 90% melanoma cell number and increases cellular NAD+, ATP, ROS levels. Nicotinamide Hydrochloride inhibts tumor growthin vivoand improves survival of melanoma-bearing mice, which can be used for the research of skin cancers such as melanoma[1].

IC50& Target

Human Endogenous Metabolite

 

SIRT2

2 μM (EC50)

体外研究
(In Vitro)

Nicotinamide Hydrochloride (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells[1].
Nicotinamide Hydrochloride (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase[1].
Nicotinamide Hydrochloride (1-50 mM, 6 h ) increases NAD+, ATP and ROS levels in A375 and SK-MEL-28 cells[1].
Nicotinamide Hydrochloride (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC50of 2 μM[1].

Cell Viability Assay[1]

Cell Line:A375, SK-MEL-28, mouse B16-F10 cell
Concentration:0, 1, 20, 50 mM
Incubation Time:24 h, 48 h
Result:Reduced cell number in a dose-dependent manner with a strong inhibitory effect at 20mM and an almost complete effect at 50 mM.

Cell Cycle Analysis[1]

Cell Line:A375, SK-MEL-28
Concentration:10, 20, 50 mM
Incubation Time:24 h
Result:Arrested A375 cells in G1 phase.
体内研究
(In Vivo)

Nicotinamide Hydrochloride (Intraperitoneal injection, 1500 and 1800 mg/Kg, 5 days per week) inhibits tumor growth in murine metastatic melanoma model[1].
Nicotinamide Hydrochloride (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10[1].

Animal Model:C57BL/6 mice (subcutaneous injected with B16-F10 cells)[1]
Dosage:1000, 1500, 1800 mg/Kg.
Administration:Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
Result:Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight.
Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.
Clinical Trial
分子量

158.59

Formula

C6H7ClN2O

CAS 号

25334-23-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.