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Ginsenoside Rd
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ginsenoside Rd图片
CAS NO:52705-93-8
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
人参皂苷 Rd
Gypenoside VIII
产品介绍
Ginsenoside Rd 抑制 TNFα 诱导的NF-κB转录活性,IC50为 12.05±0.82 μM。Ginsenoside Rd 抑制COX-2iNOSmRNA 的表达。Ginsenoside Rd 还抑制Ca2+内流。Ginsenoside Rd 抑制CYP2D6CYP1A2CYP3A4CYP2C9IC50分别为 58.0±4.5 μM,78.4±5.3 μM,81.7±2.6 μM 和 85.1±9.1 μM。
生物活性

Ginsenoside Rd inhibits TNFα-inducedNF-κBtranscriptional activity with anIC50of 12.05±0.82 μM in HepG2 cells. Ginsenoside Rd inhibits expression ofCOX-2andiNOSmRNA. Ginsenoside Rd also inhibitsCa2+influx. Ginsenoside Rd inhibitsCYP2D6,CYP1A2,CYP3A4, andCYP2C9, withIC50s of 58.0±4.5 μM, 78.4±5.3 μM, 81.7±2.6 μM, and 85.1±9.1 μM, respectively.

IC50& Target[1][2][4]

NF-κB

12.05 μM (IC50, in HepG2 cells)

COX-2

 

L-type calcium channel

 

CYP2D6

58 μM (IC50)

CYP1A2

78.4 μM (IC50)

CYP3A4

81.7 μM (IC50)

CYP2C9

85.1 μM (IC50)

体外研究
(In Vitro)

Ginsenoside Rd is one of the most abundant ingredients ofPanax ginseng. Ginsenoside Rd significantly inhibits TNF-α-induced NF-κB transcriptional activity with an IC50of 12.05±0.82 in HepG2 cells. Ginsenoside Rd also inhibits expression of COX-2 and iNOS mRNA and iNOS promoter activity in a dose-dependent manner. To determine nontoxic concentrations, HepG2 cells are treated with various concentrations (0.1, 1, and 10 μM) of compounds (e.g., Ginsenoside Rd) and cell viability is measured using an MTS assay. No compounds are significantly cytotoxic at up to 10 μM, indicating that NF-κB inhibition is not due to cell toxicity[1]. Ginsenoside Rd is one of the most abundant ingredients ofPanax ginseng, protects the heart via multiple mechanisms including the inhibition of Ca2+influx. Ginsenoside Rd reducesICa,Lpeak amplitude in a concentration-dependent manner (IC50=32.4±7.1 μM)[2]. Ginsenoside Rd exhibits an inhibition against the activity of CYP2D6 in human liver microsomes with an IC50of 58.0±4.5 μM, a weak inhibition against the activity of CYP1A2, CYP3A4, and CYP2C9 in human liver microsomes with IC50s of 78.4±5.3, 81.7±2.6, and 85.1±9.1, respectively, and an even weaker inhibition against the activity of CYP2A6 in human liver microsomes with an IC50value of more than 100 μM[4].

体内研究
(In Vivo)

Ginsenosides Rd is a major compound isolated fromGynostemma pentaphyllumthat holistically improves gut microenvironment and induces anti-polyposis in ApcMin/+mice. Six-weeks-old mice are subjected to Ginsenoside Rd treatment, before the appearance of the intestinal polyps. All the mice are monitored for food intake, water consumption, and weight changes. Throughout the experiment, no Rb3/ Ginsenoside Rd-associated weight loss in mice is observed. In addition, none of the treated mice show variations in food and water consumption. Whereas, the number and size of the polyps are effectively reduced by Ginsenoside Rd treatments[3].

分子量

947.15

性状

Solid

Formula

C48H82O18

CAS 号

52705-93-8

中文名称

人参皂苷 Rd

结构分类
  • Terpenoids
  • Triterpenes
来源
  • Plants
  • Araliaceae
  • Panax ginsengC. A. Meyer
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL(105.58 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.0558 mL5.2790 mL10.5580 mL
5 mM0.2112 mL1.0558 mL2.1116 mL
10 mM0.1056 mL0.5279 mL1.0558 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (2.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (2.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (2.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.64 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。