Bromfenac Sodium

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Bromfenac Sodium

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

91714-93-1

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
10mg	电议
50mg	电议
100mg	电议
200mg	电议
500mg	电议
1 g	电议

产品名称

溴芬酸钠

产品介绍

Bromfenac sodium 是一种强效且具有口服活性的COX抑制剂，对 COX-1 和 COX-2 的IC₅₀分别为 5.56 和 7.45 nM。Bromfenac sodium 可用于眼部炎症研究。

生物活性

Bromfenac sodium is a potent and orally active inhibitor ofCOX, withIC₅₀s of 5.56 and 7.45 nM forCOX-1andCOX-2, respectively. Bromfenac sodium can be used in ocular inflammation research^[1].

IC₅₀& Target^[1]

COX-1

5.56 nM (IC₅₀)

COX-2

7.45 nM (IC₅₀)

体外研究
(In Vitro)

Bromfenac (0-80 μg/mL; 24 h) can inhibit transforming growth factor-β2-induced epithelial-mesenchymal transition in HLEC-B3 in a concentration-dependent manner^[2].
Bromfenac (80 μg/Ml; 48 h) inhibits transforming growth factor-β2-induced epithelial-mesenchymal transition in human anterior capsules^[2].

Cell Viability Assay^[2]

Cell Line:	transforming growth factor-β2-treated human anterior capsules.
Concentration:	80 μg/mL
Incubation Time:	48 hours
Result:	Suppressed transforming growth factor-β2-induced epithelial-mesenchymal transition in primary lens epithelial cells (LECs).

Cell Migration Assay^[2]

Cell Line:	HLEC-B3 cells
Concentration:	0, 20, 40, 60, and 80 μg/mL
Incubation Time:	24 hours
Result:	Suppressed transforming growth factor-β2-induced cell migration in HLEC-B3 cells, and exhibited inhibition of the over-expression of epithelial-mesenchymal transition markers.

体内研究
(In Vivo)

Bromfenac (0.0032-3.16%; 100 or 200 μL; rubbed onto the backs) produces significant anti-inflammatory activity at concentrations as low as 0.1% (4 h pretreatment time) or 0.32% (18 h pretreatment time) in rats^[3].
Bromfenac (0.032-3.16%; 100 μL; rubbed onto the paws) produces dose-related anti-inflammatory activity in rats^[3].
Bromfenac (0.032-1.0%; 50 μL) is 26 times more potent than indomethacin in blocking the erythema when applied directly onto the skin area exposed to UV light in guinea pigs^[3].
Bromfenac (0.0032-0.1%; 50 μL; rubbed onto the uninjected paw for 4 h per day and 5 days per week) produces a dose and time dependent reduction in the paw volume of both hind limbs in rats^[3].
Bromfenac (0.32%; 50 μL; rubbed onto the abdomen) produces significant blockade of abdominal constriction to ACh challenge in mice^[3].
Bromfenac (eyedrop instillation; 1 μL (0.09%) per eye; twice-daily; 4 w) partially reduces corneal staining, and becomes so more slowly by the 4-week time point^[4].

Animal Model:	Male Sprague-Dawley rats (150-250 g) are injected carrageenan^[2]
Dosage:	0.0032, 0.01, 0.032, 0.1, 0.32, 1.0, 3.16% (100 or 200 μL)
Administration:	Rubbed onto the backs before 1-72 h of injected carrageenan
Result:	Produced significant anti-inflammatory activity when applied 1, 2, and 4 h prior to carrageenan challenge at 0.32%. Applied 1 or 4 h prior to carrageenan challenge was active, but not when applied 24 h (or longer) prior to challenge at 0.2%.

Clinical Trial

分子量

356.15

性状

Solid

Formula

C₁₅H₁₁BrNNaO₃

CAS 号

91714-93-1

中文名称

溴苯酸钠

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

DMSO : 11.36 mg/mL(31.90 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.8078 mL	14.0390 mL	28.0781 mL
5 mM	0.5616 mL	2.8078 mL	5.6156 mL
10 mM	0.2808 mL	1.4039 mL	2.8078 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline
Solubility: ≥ 1.14 mg/mL (3.20 mM); Clear solution
此方案可获得 ≥ 1.14 mg/mL (3.20 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 11.4 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
2.
请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline)
Solubility: ≥ 1.14 mg/mL (3.20 mM); Clear solution
此方案可获得 ≥ 1.14 mg/mL (3.20 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 11.4 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在本网站选购。