COX-2-IN-10 是一种有效的COX-2抑制剂。COX-2-IN-10 以浓度依赖性方式抑制 PGE2的产生 (IC50=2.54 μM)。COX-2-IN-10 在 mRNA 和蛋白水平上抑制iNOS和COX-2的表达。COX-2-IN-10 抑制 IL-6、TNF-α 和 IL-1β 的产生。
| 生物活性 | COX-2-IN-10 is a potentCOX-2inhibitor. COX-2-IN-10 inhibits the production of PGE2in concentration dependent manner (IC50=2.54 μM). COX-2-IN-10 inhibits the expression ofiNOSandCOX-2on mRNA and protein level . COX-2-IN-10 inhibits the production ofIL-6, TNF-α and IL-1β[1]. |
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体外研究
(In Vitro) | COX-2-IN-10 (compound 9k) (10 μM, 24 h) exhibits high safety and did not show any significant cytotoxicity over LPS-Induced RAW264.7 macrophage cells[1].
COX-2-IN-10 (0.4, 2, 10 μM) inhibits the production of PGE2in concentration dependent manner in RAW264.7 macrophage cells (IC50=2.54 μM)[1].
COX-2-IN-10 (2.5, 5, 10 μM) significantly inhibits the mRNA expression of iNOS and COX-2 at 10 μM[1].
COX-2-IN-10 (2.5, 5, 10 μM) significantly inhibits iNOS and COX-2 protein level starting from 10 μM concentration[1].
COX-2-IN-10 (2.5, 5, 10 μM; 1 h) inhibits the production of IL-6, TNF-α and IL-1β[1].
Cell Viability Assay[1] | Cell Line: | RAW264.7 macrophage cells | | Concentration: | 10 μM | | Incubation Time: | 24 h | | Result: | Exhibited high safety and did not show any significant cytotoxicity over LPS-Induced RAW264.7 macrophage cells. |
RT-PCR[1] | Cell Line: | RAW264.7 macrophage cells | | Concentration: | 2.5, 5, 10 μM | | Incubation Time: | | | Result: | Inhibits the mRNA expression of iNOS and COX-2 at 10 μM. |
Western Blot Analysis[1] | Cell Line: | RAW264.7 macrophage cells | | Concentration: | 2.5, 5, 10 μM | | Incubation Time: | | | Result: | Significantly inhibited iNOS and COX-2 protein level starting from 10 μM concentration. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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