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PACAP-38(31-38),human,mouse,rat
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
PACAP-38(31-38),human,mouse,rat图片
CAS NO:138764-85-9
包装与价格:
包装价格(元)
500 μg电议
1mg电议
5mg电议
10mg电议
50mg电议
100mg电议

产品介绍
PACAP-38 (31-38), human, mouse, rat 是一种PAC1受体激活剂,增加 α-分泌酶活性。PACAP-38 (31-38), human, mouse, rat 升高胞浆 Ca2+,增加细胞外调节激酶 (ERK) 和表皮生长因子受体 (EGFR) 的增殖和磷酸化。PACAP-38 (31-38), human, mouse, rat 对交感神经 NPY 和儿茶酚胺的产生具有强效、持续的刺激作用。PACAP-38 (31-38), human, mouse, rat 可用于神经营养和神经保护研究。
生物活性

PACAP-38 (31-38), human, mouse, rat is aPAC1receptoractivator and increases the α-secretase activity. PACAP-38 (31-38), human, mouse, rat elevates cytosolic Ca2+, increases proliferation and increases phosphorylation of extracellular regulates kinase (ERK) and the epidermal growth factor receptor (EGFR). PACAP-38 (31-38), human, mouse, rat demonstrates potent, efficacious, and sustained stimulatory effects on sympathetic neuronal NPY and catecholamine production. PACAP-38 (31-38), human, mouse, rat can be used for neurotrophic and neuroprotective research[1][2][3].

体外研究
(In Vitro)

PACAP-38 (31-38), human, mouse, rat (100 nM; 2 min; NCI-H838 cells) induces EGFR, HER2 and ERK tyrosine phosphorylation[1].
PACAP-38 (31-38), human, mouse, rat (100 nM; 30 min; NCI-H838 cells) induces EGFR tyrosine phosphorylation with generates ROS and increases ROS levels by 51%[1].
PACAP-38 (31-38), human, mouse, rat (10 nM; 48 h) stimulates the growth of NCI-H838 cells[1].
PACAP-38 (31-38), human, mouse, rat (300 nM; 4 h) stimulates generation of APPsα in neural cells[2].
PACAP-38 (31-38), human, mouse, rat (0.01-10 nM; HEK 293 cells) stimulates neural cells express endogenous PAC1 receptors by cAMP accumulation and by an increase in cytosolic free calcium and induces elevation of the intracellular Ca2+concentration in a dose-dependent manner with an EC50value of 0.81 nM[2].
PACAP-38 (31-38), human, mouse, rat (0.01 nM; 48 h) elicits potent and efficacious stimulation of NPY secretion from SCG neuronal cultures[3].
PACAP-38 (31-38), human, mouse, rat (100 nM; 14 d) produces sustained stimulated NPY and catecholamine secretionmpathetic neurons[3].

Cell Viability Assay[1]

Cell Line:NCI-H838 cells
Concentration:10 nM
Incubation Time:48 hours
Result:Increased the number of NCI-H838 cells by 72%.

Western Blot Analysis[1]

Cell Line:100 nM
Concentration:100 nM
Incubation Time:2 minutes
Result:Increased tyrosine phosphorylation of the EGFR, HER2, and ERK by 377, 299 and 216%, respectively.
分子量

1062.27

性状

Solid

Formula

C47H83N17O11

CAS 号

138764-85-9

Sequence

Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2

Sequence Shortening

YKQRVKNK-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 100 mg/mL(94.14 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM0.9414 mL4.7069 mL9.4138 mL
5 mM0.1883 mL0.9414 mL1.8828 mL
10 mM0.0941 mL0.4707 mL0.9414 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。