PACAP-38 (31-38), human, mouse, rat TFA 是一种 PAC1受体激活剂。PACAP-38 (31-38), human, mouse, rat TFA 增加 α-分泌酶活性。PACAP-38 (31-38), human, mouse, rat 升高胞浆 Ca2+，增加细胞外调节激酶 (ERK) 和表皮生长因子受体 (EGFR) 的增殖和磷酸化。PACAP-38 (31-38), human, mouse, rat TFA 对交感神经 NPY 和儿茶酚胺的产生具有强效、持续的刺激作用。PACAP-38 (31-38), human, mouse, rat TFA 可用于神经营养和神经保护研究。
| 生物活性 | PACAP-38 (31-38), human, mouse, rat TFA is aPAC1receptoractivator and increases the α-secretase activity. PACAP-38 (31-38), human, mouse, rat TFA elevates cytosolic Ca2+, increases proliferation and increases phosphorylation of extracellular regulates kinase (ERK) and the epidermal growth factor receptor (EGFR). PACAP-38 (31-38), human, mouse, rat TFA demonstrates potent, efficacious, and sustained stimulatory effects on sympathetic neuronal NPY and catecholamine production. PACAP-38 (31-38), human, mouse, rat TFA can be used for neurotrophic and neuroprotective research[1][2][3]. |
体外研究
(In Vitro) | PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 2 min; NCI-H838 cells) induces EGFR, HER2 and ERK tyrosine phosphorylation[1].
PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 30 min; NCI-H838 cells) induces EGFR tyrosine phosphorylation with generates ROS and increases ROS levels by 51%[1].
PACAP-38 (31-38), human, mouse, rat TFA (10 nM; 48 h) stimulates the growth of NCI-H838 cells[1].
PACAP-38 (31-38), human, mouse, rat TFA (300 nM; 4 h) stimulates generation of APPsα in neural cells[2].
PACAP-38 (31-38), human, mouse, rat TFA (0.01-10 nM; HEK 293 cells) stimulates neural cells express endogenous PAC1 receptors by cAMP accumulation and by an increase in cytosolic free calcium and induces elevation of the intracellular Ca2+concentration in a dose-dependent manner with an EC50value of 0.81 nM[2].
PACAP-38 (31-38), human, mouse, rat TFA (0.01 nM; 48 h) elicits potent and efficacious stimulation of NPY secretion from SCG neuronal cultures[3].
PACAP-38 (31-38), human, mouse, rat TFA (100 nM; 14 d) produces sustained stimulated NPY and catecholamine secretionmpathetic neurons[3].
Cell Viability Assay[1] | Cell Line: | NCI-H838 cells | | Concentration: | 10 nM | | Incubation Time: | 48 hours | | Result: | Increased the number of NCI-H838 cells by 72%. |
Western Blot Analysis[1] | Cell Line: | NCI-H838 cells | | Concentration: | 100 nM | | Incubation Time: | 2 min | | Result: | Increased tyrosine phosphorylation of the EGFR, HER2, and ERK by 377, 299 and 216%, respectively. |
|
| 分子量 | |
| 性状 | |
| Formula | |
| Sequence | Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2 |
| Sequence Shortening | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Sealed storage, away from moisture | Powder | -80°C | 2 years | | -20°C | 1 year |
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : 100 mg/mL(85.01 mM;Need ultrasonic) 配制储备液 | 1 mM | 0.8501 mL | 4.2507 mL | 8.5013 mL | | 5 mM | 0.1700 mL | 0.8501 mL | 1.7003 mL | | 10 mM | 0.0850 mL | 0.4251 mL | 0.8501 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 100 mg/mL (85.01 mM); Clear solution; Need ultrasonic
*以上所有助溶剂都可在本网站选购。 |
m.cnreagent.com