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Ascochlorin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ascochlorin图片
CAS NO:26166-39-2
包装与价格:
包装价格(元)
500 μg电议
1mg电议

产品名称
Ilicicolin D
产品介绍
Ascochlorin (Ilicicolin D),异戊二烯类抗生素。Ascochlorin 主要通过抑制STAT3信号级联介导其抗肿瘤作用。Ascochlorin 诱导细胞凋亡 (apoptosis)。具有抗炎活性。
生物活性

Ascochlorin (Ilicicolin D), an isoprenoidantibiotic, mediates its anti-tumor effects predominantly through the suppression ofSTAT3signaling cascade. Ascochlorin inducesapoptosis. Anti-inflammatory activity[1][2][3].

IC50& Target[3]

STAT3

 

Apoptosis

 

体外研究
(In Vitro)

Ascochlorin (Ilicicolin D) (10-50 μM; 24-72 hours) inhibits the viability of HepG2, HCCLM3 and Huh7 cells in a time and dose dependent manner[3].
Ascochlorin (50 μM; 48 hours) induces apoptosis in HCC cells[3].
Ascochlorin (1-50 μM) significantly suppresses the production of nitric oxide (NO) and prostaglandin E2 (PGE2) and decreases the gene expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner. Ascochlorin inhibits the mRNA expression and the protein secretion of interleukin (IL)-1β and IL-6 but not tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 macrophage cells. Ascochlorin suppresses nuclear translocation and DNA binding affinity of nuclear factor-κB (NF-κB). Ascochlorin down-regulates phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) and p-p38[2].

Cell Viability Assay[3]

Cell Line:HepG2, HCCLM3, Huh7 cells
Concentration:10, 25, 50 μM
Incubation Time:24, 48, 72 hours
Result:Inhibit the viability of three different HCC cell lines tested (HepG2, HCCLM3 and Huh7) in a time and dose dependent manner.

Western Blot Analysis[3]

Cell Line:HepG2 cells
Concentration:50 μM
Incubation Time:48 hours
Result:Inhibited expression of the cell cycle regulator protein cyclin D1, the anti-apoptotic proteins Bcl-2, Mcl-1, survivin and XIAP, and the invasive gene product MMP-9.
体内研究
(In Vivo)

Ascochlorin (Ilicicolin D) (2.5-5 mg/kg; i.p.; day 0, 1, 2, 3, 13, 15, 17, 20, 22, 24, 27, 29 and 31) inhibits tumor growth in an orthotopic HCC mouse model[1].

Animal Model:Eight week-old athymic balb/c nude female mice (HCCLM3-Luc2 tumors)[3]
Dosage:2.5 mg/kg, 5 mg/kg
Administration:I.p.; day 0, 1, 2, 3, 13, 15, 17, 20, 22, 24, 27, 29 and 31
Result:Induced significant inhibition of tumor growth.
分子量

404.93

Formula

C23H29ClO4

CAS 号

26166-39-2

结构分类
  • Phenols
  • Polyphenols
  • Antibiotics
  • Other Antibiotics
来源
  • Plants
  • other families

Ascochyta viciae

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.