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Lidocaine hydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Lidocaine hydrochloride图片
CAS NO:73-78-9
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
500mg电议
5 g电议
10 g电议
50 g电议

产品名称
盐酸利多卡因
Lignocaine hydrochloride
产品介绍
Lidocaine (Lignocaine) hydrochloride 抑制涉及复杂电压和依赖性的钠通道 (sodium channels)。Lidocaine hydrochloride 通过调节 miR-145 表达和进一步抑制MEK/ERKNF-κB信号通路来减少胃癌细胞的生长,迁移和侵袭。Lidocaine 是一种酰胺衍生物,是一种研究室性心律失常的药物和有效的肿瘤抑制剂。
生物活性

Lidocaine hydrochloride (Lignocaine hydrochloride) inhibitssodium channelsinvolving complex voltage and using dependence[1]. Lidocaine hydrochloride decreases growth, migration and invasion of gastric carcinoma cells via up-regulating miR-145 expression and further inactivation ofMEK/ERKandNF-κBsignaling pathways. Lidocaine hydrochloride is an amide derivative and a drug to treat ventricular arrhythmia and an effective tumor-inhibitor[2].

IC50& Target[1,2]

MEK

 

ERK

 

NF-κB

 

体外研究
(In Vitro)

Lidocaine hydrochloride (Lignocaine hydrochloride) (10 nM; 48 hours) decreases significantly cell proliferation[2].
Lidocaine hydrochloride (1-10 nM; 24-72 hours) inhibits cell viability and achieves the most suppressing effects at the concentration of 10 nM and treatment time 48 hours[2].
Lidocaine hydrochloride (10 nM; 48 hours) increases significantly the apoptotic cell rate[2].
Lidocaine hydrochloride (10 nM; 48 hours) down-regulates Cyclin D1 and up-regulates p21 expression significantly[2].

Cell Proliferation Assay[2]

Cell Line:The human gastric cancer cell line MKN45
Concentration:10 nM
Incubation Time:48 hours
Result:Decreased significantly cell proliferation.

Cell Viability Assay[2]

Cell Line:The human gastric cancer cell line MKN45
Concentration:1, 5 and 10 nM
Incubation Time:24, 48, 72 hours
Result:Inhibited MKN45 cell viability.

Apoptosis Analysis[2]

Cell Line:The human gastric cancer cell line MKN45
Concentration:10 nM
Incubation Time:48 hours
Result:Increased significantly the apoptotic cell rate.

Western Blot Analysis[2]

Cell Line:The human gastric cancer cell line MKN45
Concentration:10 nM
Incubation Time:48 hours
Result:Down-regulated Cyclin D1 and up-regulated p21 expression significantly.
体内研究
(In Vivo)

Lidocaine hydrochloride (Lignocaine hydrochloride) causes completely reversible tail nerve block in rats. Mechanical nociception block produced by lidocaine has slower onset and faster recovery compared with thermal nociception block[3].

Clinical Trial
分子量

270.80

性状

Solid

Formula

C14H23ClN2O

CAS 号

73-78-9

中文名称

盐酸利多卡因

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

H2O : ≥ 100 mg/mL(369.28 mM)

DMSO : ≥ 100 mg/mL(369.28 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.6928 mL18.4638 mL36.9276 mL
5 mM0.7386 mL3.6928 mL7.3855 mL
10 mM0.3693 mL1.8464 mL3.6928 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 120 mg/mL (443.13 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (9.23 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.23 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (9.23 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.23 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 4.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (9.23 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.23 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。