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DMU-212
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
DMU-212图片
CAS NO:134029-62-2
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议

产品介绍
DMU-212 是一种白藜芦醇 (HY-16561) 甲基化衍生物,具有抗分裂、抗增殖、抗氧化和促进细胞凋亡的活性。DMU-212 通过诱导凋亡 (apoptosis) 和激活ERK1/2蛋白导致有丝分裂停止。DMU-212 具有口服活性。
生物活性

DMU-212 is a methylated derivative of Resveratrol (HY-16561), with antimitotic, anti-proliferative, antioxidant andapoptosispromoting activities. DMU-212 induces mitotic arrest via induction ofapoptosisand activation ofERK1/2protein. DMU-212 has orally active[1][2].

体外研究
(In Vitro)

DMU-212 (0.3125-40 μM) inhibits growth of A375, MeWo, Bro and M5 human melanoma cells[1].
DMU-212 (30-50 μM; 24 hours) induces upregulation of cell cycle inhibitors, apoptosis and ERK activation in A375 cells[1].

Cell Proliferation Assay[1]

Cell Line:A375 cells, MeWo cells, M5 cells, Bro cells
Concentration:0.3125 μM, 0,625 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM, 20 μM, 40 μM
Incubation Time:96 hours
Result:Inhibited the cellular proliferation of human melanoma cells at submicromolar or micromolar concentrations (IC50=0.5 μM for A375 and Bro and IC50= 1.25 μM for MeWo and M5 cells).

Cell Cycle Analysis[1]

Cell Line:A375 cells
Concentration:20 μM, 30 μM, 50 μM
Incubation Time:24 hours
Result:Caused a marked increase in the levels of p21, p53 and cyclin B1 proteins with a concomitant decrease in the levels of cyclin A2.

Western Blot Analysis[1]

Cell Line:A375 cells
Concentration:20 μM, 30 μM, 50 μM
Incubation Time:24 hours
Result:Significant upregulated Bax, caspase 3 and caspase 9 protein levels, while decreased the levels of the anti-apoptotic protein Bcl-2.

Apoptosis Analysis[1]

Cell Line:A375 cells
Concentration:10 μM, 20 μM
Incubation Time:24 hours, 36 hours
Result:Induced apoptosis.
体内研究
(In Vivo)

DMU-212 (50 mg/kg; i.g.; three times a week; for 14 days) inhibits tumor growth in xenograft model of human ovarian cancer[2].

Animal Model:6-weeks-old SCID female mice (20-24 g), with ovarian cancer xenografts[2]
Dosage:50 mg/kg
Administration:Oral gavage, three times a week, for 14 days
Result:Lowered tumor burden.
分子量

300.35

性状

Solid

Formula

C18H20O4

CAS 号

134029-62-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 20 mg/mL(66.59 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.3294 mL16.6472 mL33.2945 mL
5 mM0.6659 mL3.3294 mL6.6589 mL
10 mM0.3329 mL1.6647 mL3.3294 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2 mg/mL (6.66 mM); Clear solution

    此方案可获得 ≥ 2 mg/mL (6.66 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。