Cearoin 通过促进ROS产生和激活ERK来增强自噬 (autophagy) 和诱导细胞凋亡(apoptosis)。
| 生物活性 | Cearoin increasesautophagyandapoptosisthrough the production ofROSand the activation ofERK[1]. |
| IC50& Target[1] | ERK | ROS | Autophagy | Apoptosis |
|
体外研究
(In Vitro) | Cearoin (10-80 μM) induces cell death in a dose-dependent manner[1].
Cearoin (10-80 μM) increases the phosporylation of ERK in SH-SY5Y cells[1].
Cearoin (5-80 μM) increases the conversion of LC3B-I to LC3B-II in SH-SY5Y cells. The expression of LC3B-II is a good marker for autophagosome formation in the autophagy process[1].
Cell Viability Assay[1] | Cell Line: | Human neuroblastoma SH-SY5Y cells | | Concentration: | 0, 1, 5, 10, 20, 40, or 80 μM | | Incubation Time: | 6 or 12 hours | | Result: | Significantly decreased cell viability from 10 μM in a dose-dependent manner. Treatment with 40 μM for 12 h induced about 50% loss in cell viability in SH-SY5Y cells. |
Western Blot Analysis[1] | Cell Line: | Human neuroblastoma SH-SY5Y cells | | Concentration: | 0, 5, 10, 20, 40, or 80 μM | | Incubation Time: | 12 hours | | Result: | Increased ERK phosphorylation in a dose-dependent manner, whereas it did not alter JNK phosphorylation.
Induced the formation of LC3B-II in a dose dependent manner. |
|
| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| 结构分类 | - Ketones, Aldehydes, Acids
|
| 来源 | - Plants
- Leguminosae
- Dalbergia odoriferaT. Chen
|
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 50 mg/mL(204.72 mM;Need ultrasonic) 配制储备液 | 1 mM | 4.0943 mL | 20.4717 mL | 40.9433 mL | | 5 mM | 0.8189 mL | 4.0943 mL | 8.1887 mL | | 10 mM | 0.4094 mL | 2.0472 mL | 4.0943 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
m.cnreagent.com