Withanolide B 是W. somnifera Dunal的活性成分。Withanolide B 通过ERK1/2和Wnt/β-catenin信号通路促进 hBMSCs 的成骨分化。 Withanolide B 具有神经保护、抗关节炎、抗衰老和抗癌的作用。
| 生物活性 | Withanolide B is an active component ofW. somnifera Dunal. Withanolide B promotes osteogenic differentiation of hBMSCs viaERK1/2andWnt/β-catenin signaling pathways. Withanolide B exhibits neuroprotective, anti-arthritic,anti-agingand anti-cancer effects[1][2][3]. |
体外研究
(In Vitro) | Withanolide B (1-100 nM; 3-5 days) significantly increases the expression of COL1A1 and RUNX2 genes and proteins in hBMSCs[1].
Withanolide B (1-100 nM; 11 or 3 days) increases the formation of extracellular matrix calcium deposits and increased the activity of alkaline phosphatase (ALP)[1].
Withanolide B (1-100 nM; 3-5 days) increases the protein expressions of p-ERK and active β-catenin of hBMSCs[1].
Withanolide B (10-100 μM; 48 h) reverses the Aβ42 aggregation-induced toxicy in SK-N-SH cells[2].
Cell Viability Assay[1] | Cell Line: | Human bone mesenchymal stem cells | | Concentration: | 1, 10, 100 nM | | Incubation Time: | 3, 5 days | | Result: | Significantly increased the expression of COL1A1 and RUNX2. |
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体内研究
(In Vivo) | Withanolide B (10 mg/kg; topical administration) promotes bone healing in a rat tibial defect model[1].
| Animal Model: | Male Sprague-Dawley rats (8 weeks, 200 g) with tibial defect[1] | | Dosage: | 10 mg/kg | | Administration: | Injected in situ at the bone defect site at different time points (0, 3, 5, 7, and 9 days) | | Result: | Increased the trabecular number, trabecular thickness and the thickness of the cortical bone. |
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| 来源 | - Plants
- Solanaceae
- Withania somnifera
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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