SY-LB-35 是一种有效的骨形态发生蛋白 (BMP) 受体激动剂。SY-LB-35 能刺激 C2C12 成肌细胞显著增加细胞数量和细胞活力，使细胞周期向 S 期和 G2/M 期转变。SY-LB-35 刺激典型的Smad和非典型的PI3K/Akt、ERK、p38和JNK胞内信号通路。
| 生物活性 | SY-LB-35 is a potentbone morphogenetic protein(BMP) receptoragonist. SY-LB-35 can stimulate significant increases in cell number and cell viability in the C2C12 myoblast cell line, and causes shifts towards the S and G2/M phases of the cell cycle. SY-LB-35 stimulates canonicalSmadand non-canonicalPI3K/Akt,ERK,p38andJNKintracellular signaling pathways[1]. |
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体外研究
(In Vitro) | SY-LB-35 (0.01-1000 μM; 24 h) stimulates significant increases in cell number and cell viability in the C2C12 myoblast cell line[1].
SY-LB-35 (0.01-10 μM; 30 min or 15 min) stimulates Smad phosphorylation and nuclear translocation, activates the PI3K/Akt pathway and direct the cytoplasmic localization of p-Akt, stimulates the phosphorylation and activation of PI3K in the C2C12 cells[1].
SY-LB-35 (0.01-10 μM; 24 h) causes the cell cycle shifting to S and G2/M phases in the C2C12 cells[1].
Cell Viability Assay[1] | Cell Line: | C2C12 cells | | Concentration: | 0.01, 0.1, 1, 10, 100 and 1000 μM | | Incubation Time: | 24 h | | Result: | Stimulated significant increases in cell number and cell viability. |
Western Blot Analysis[1] | Cell Line: | C2C12 cells | | Concentration: | 0.01, 0.1, 1 and 10 μM | | Incubation Time: | 30 or 15 min | | Result: | Stimulated Smad phosphorylation and nuclear translocation, activated the PI3K/Akt pathway and direct the cytoplasmic localization of p-Akt, stimulated the phosphorylation and activation of PI3K in the C2C12 cells. |
Cell Cycle Analysis[1] | Cell Line: | C2C12 cells | | Concentration: | 0.01, 0.1, 1 and 10 μM | | Incubation Time: | 24 h | | Result: | Caused the cell cycle shifting to S and G2/M phases. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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