Anti-inflammatory agent 31 是一种穿心莲内酯衍生物,是一种抗炎剂 (anti-inflammatoryagent)。Anti-inflammatory agent 31 通过上游阻断PI3K/Akt和ERK1/2 MAPK激活来抑制NF-kB激活。Anti-inflammatory agent 31 能够恢复细胞内 GSH 水平并对肝脏具有保护作用。
生物活性 | Anti-inflammatory agent 31 (enone 17) is a kind of andrographolide derivatives, is aanti-inflammatoryagent. Anti-inflammatory agent 31 inhibitsNF-κBactivation by upstream blockade ofPI3K/AktandERK1/2 MAPKactivation. Anti-inflammatory agent 31 shows recovery effective of the intracellular GSH levels and protective effect on liver[1]. |
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体外研究 (In Vitro) | Anti-inflammatory agent 31 (enone 17) (100, 200, and 400 μM; 24 h) prevents the DNA binding of the activated NF-κB p50 subunit in a dose-dependent manner[1]. Anti-inflammatory agent 31 reacts as a quick rate (KGSH=0.076 M-1.h-1) and long half-live (T1/2=6.47 h)[1]. Anti-inflammatory agent 31 (5, 20, and 50 μM; 24 h) shows recovery effective of the intracellular GSH levels, and (5 μM) induced more than 2.5-fold increase in GSH levels as compared to the basal levels[1]. Anti-inflammatory agent 31 (5, 20, and 50 μM; 24 h) displays low cytotoxicity in LO2 and HEK293 cells with CC50of 14.68, 26.25 μM, respectively[1].
Immunofluorescence[1] Cell Line: | RAW-blueTMcells | Concentration: | 5, 10, 20 μM | Incubation Time: | 24 hours | Result: | Inhibited the LPS-induced NF-κB activity and indicated the anti-inflammatory properties. |
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体内研究 (In Vivo) | Anti-inflammatory agent 31 (enone 17) (i.p.; 0.3, 1.0 mg/kg) decreases total white blood cell and neutrophils counts, and alleviates LPS-induced acute lung injury in a dose-dependent manner in mouse[1].
Animal Model: | LPS-induced acute lung injury (ALI) mouse model (female BALB/c mice; 7-week-old)[1] | Dosage: | 0.3, 1.0 mg/kg | Administration: | Intraperitoneal injection 1 h after LPS challenge; twice daily for 4 days | Result: | Alleviated LPS-induced acute lung injury. |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |