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DCDAPH
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
DCDAPH图片
CAS NO:125113-96-4
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
DCDAPH (Compound 2c) 是一种新型的智能近红外探针,用于检测 β-淀粉样蛋白 () 斑块 (在 PBS 中 λexem=597/665 nm)。DCDAPH 对 Aβ 表现出高亲和力 (Ki=37 nM,Kd=27 nM)。DCDAPH 具有良好的血脑屏障渗透性并可满足在体外和体内 Aβ 检测的大部分要求。
生物活性

DCDAPH (Compound 2c) is a novel smart NIRF probe for detection ofβ-amyloid() plaques (λexem=597/665 nm in PBS). DCDAPH shows high affinity for Aβ aggregates (Ki=37 nM,Kd=27 nM). DCDAPH shows good blood brain barrier permeation and can meet most of the requirements for the detection of Aβ plaques both in vitro and in vivo[1].

体外研究
(In Vitro)

DCDAPH (0-10 μM; 24 h) treatment shows no toxicity to human neuronal cells[1].

Cell Viability Assay[1]

Cell Line:SH-SY5Y cells
Concentration:0, 0.1, 1, and 10 μM
Incubation Time:24 hours
Result:Showed no marked toxicity to this human neuronal cell line at 10 μM.
体内研究
(In Vivo)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fluorescent Staining of DCDAPH to Aβ Plaques in Mouse Brain[1].
1. Mouse should be i.v. injected with DCDAPH (0.4 mg/kg, 20%DMSO(HY-Y0320), 80%propylene glycol(HY-Y0921), 50 μL), and sacrificed at 30 min after injection.
2. The brains should be excised, embedded in optimum cutting temperature compound (OCT), and frozen in powdered dry ice immediately.
3. Frozen sections of 20 μm should be cut.
4. Fluorescent observation (Cy5 filter set).

分子量

249.31

Formula

C16H15N3

CAS 号

125113-96-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.