您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Isobavachalcone
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Isobavachalcone
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Isobavachalcone图片
CAS NO:20784-50-3
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品名称
补骨脂乙素
Corylifolinin
Isobacachalcone
产品介绍
Isobavachalcone (Corylifolinin) 来源于补骨脂,是一种Akt信号通路的有效抑制剂,可诱导人类癌细胞凋亡 (以IC50值为 7.92 μM 抑制 OVCAR-8 癌细胞生长),具有抗癌和抗增殖活性。Isobavachalcone 还能诱导 OVCAR-8 细胞中活性氧的产生。
生物活性

Isobavachalcone (Corylifolinin) is derived fromPsoralea corylifolia Linn.and is a potent inhibitor ofAktsignaling pathway, which inducesapoptosisin humancancercells (Inhibits OVCAR-8 cell growth with anIC50value of 7.92 μM). Isobavachalcone also inducesReactive Oxyen Species (ROS)generation in OVCAR-8 cells and has exhibitcanceranti-promotive and anti-proliferative activity[1].

IC50& Target

IC50: 7.92 μM (OVCAR-8 cell)[1]

体外研究
(In Vitro)

Isobavachalcone (6.0-48.0 μM; 72 hours; OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells) treatment inhibits the proliferation of human cancer cells. Isobavachalcone inhibits PC3, A549, MCF-7, L-02 and HUVEC cells growth with IC50 values of 15.06 μM, 32.2 μM, 28.29 μM, 31.61 μM and 31.3 μM, respectively[1].
Isobavachalcone (0-18 μM; 6 hours; OVCAR-8 and PC3 cells) treatment results in a concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt and GSK3b phosphorylation[1].
Isobavachalcone (0-18 μM; 72 hours; OVCAR-8 and PC3 cells) treatment causes apoptosis via caspase- and ROS-involved mitochondrial pathway[1].

Cell Proliferation Assay[1]

Cell Line:OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells
Concentration:6.0-48.0 μM
Incubation Time:72 hours
Result:Inhibited the proliferation of human cancer cells.

Western Blot Analysis[1]

Cell Line:OVCAR-8 or PC3 cells
Concentration:0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:6 hours
Result:A concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt. GSK3b phosphorylation was also inhibited in a concentration- and time-dependent manner.

Apoptosis Analysis[1]

Cell Line:OVCAR-8 cells and PC3 cells
Concentration:0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:72 hours
Result:Led to dose dependent increase of apoptosis.
体内研究
(In Vivo)

Isobavachalcone (20 mg/kg; intraperitoneal injection; for 0.5-24 hours; female Kunming mice) treatment results in an increase in blood glucose levels, reaching a maximum within 1 hour and maintaining until 4 hours post-dosing[1].

Animal Model:Seven-week-old specific pathogen-free female Kunming mice (18-22 g)[1]
Dosage:20 mg/kg
Administration:Intraperitoneal injection; for 0.5, 1, 2, 4, 6, 8, 12, 24 hours
Result:Increased in blood glucose levels.
分子量

324.37

性状

Solid

Formula

C20H20O4

CAS 号

20784-50-3

中文名称

补骨脂乙素;补骨酯乙素;异补骨脂查耳酮;异补骨脂查尔酮

结构分类
  • Flavonoids
  • Chalcones
  • Phenols
  • Polyphenols
来源
  • Plants
  • Leguminosae
  • Psoralea corylifoliaL.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 11 mg/mL(33.91 mM;Need ultrasonic and warming)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.0829 mL15.4145 mL30.8290 mL
5 mM0.6166 mL3.0829 mL6.1658 mL
10 mM0.3083 mL1.5414 mL3.0829 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。