TMBIM6 antagonist-1

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TMBIM6 antagonist-1

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

123134-61-2

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
50mg	电议
100mg	电议

产品介绍

TMBIM6 antagonist-1 是一个有潜力的TMBIM6拮抗剂，抑制 TMBIM6 与 mTORC2 结合，降低 mTORC2 活性，调节TMBIM6 释放 Ca²⁺。

生物活性

TMBIM6 antagonist-1, a potentialTMBIM6antagonist, prevents TMBIM6 binding tomTORC2, decreasesmTORC2activity, and also regulates TMBIM6-leaky Ca²⁺^[1].

体外研究
(In Vitro)

TMBIM6 antagonist-1 (BIA, 0.5-10 μM, 3 days) significantly and dose-dependently inhibits cell viability in HT1080, MCF7, MDA-MB-2341 and SKBR3 cells, with IC₅₀values of 1.7 ± 0.1 μM for HT1080, 2.6 ± 0.4 μM for MCF cells, 2.6 ± 0.5 μM for MDA-MB-231 cells, and 2.4 ± 0.4 μM for SKBR3 cells, respectively^[1].
TMBIM6 antagonist-1 (BIA, 10 μM) treatment decreases cell migration in HT1080, MCF7, MDA-MB-231, and SKBR3 cells, not TMBIM6 KO HT1080 cells^[1].

Cell Viability Assay^[1]

Cell Line:	HT1080, MCF7, MDA-MB-2341 and SKBR3 cells.
Concentration:	0.5-10 μM.
Incubation Time:	3 days.
Result:	Inhibited cell viability.

Western Blot Analysis^[1]

Cell Line:	WT and TMBIM6 KO HT1080 cells.
Concentration:	0, 2, 5 μM.
Incubation Time:
Result:	Downregulated the protein levels of AKT-pS473.

体内研究
(In Vivo)

TMBIM6 antagonist-1 (1 mg/kg, IP 5 days per week during 25 days) significantly impaires cell-driven tumor growth^[1].

Animal Model:	Six- to eight-week BklNbt:BALB/c/nu/nu old mice (HT1080 and MDA-MB-231 cells)^[1].
Dosage:	1 mg/kg.
Administration:	IP 5 days per week during 25 days.
Result:	Impaired cell-driven tumor growth.

分子量

268.27

性状

Solid

Formula

C₁₅H₁₂N₂O₃

CAS 号

123134-61-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 50 mg/mL(186.38 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.7276 mL	18.6379 mL	37.2759 mL
5 mM	0.7455 mL	3.7276 mL	7.4552 mL
10 mM	0.3728 mL	1.8638 mL	3.7276 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。