8-Aminoadenosine (8-NH2-Ado) 是一种 RNA 导向的核苷类似物，可降低细胞 ATP 水平并抑制mRNA合成。8-Aminoadenosine 阻断Akt/mTOR信号，并诱导 p53 非依赖性的自噬和细胞凋亡。8-Aminoadenosine 具有抗肿瘤活性。
| 生物活性 | 8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibitsmRNA synthesis. 8-Aminoadenosine blocksAkt/mTORsignaling and inducesautophagyandapoptosisin a p53-independent manner. 8-Aminoadenosine has antitumor activity[1][2][3]. |
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体外研究
(In Vitro) | 8-Aminoadenosine (8-NH2-Ado; 0.1-10 μM; for 48 h) has IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively[1].
8-Aminoadenosine (10 μM; for 24 h) induces significant apoptotic death of MCF-7 cells in p53-independent pathway. 8-Aminoadenosine causes PARP cleavage in MCF-7 cells[2].
8-Aminoadenosine (3 μM; 0.5-4 h) induces autophagy in the MM.1S cell line[1].
8-Aminoadenosine (3 μM; 2-16 h) causes a greater drop in ATP levels in the MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) causes a 50% reduction in glucose consumption in MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) indicates a time-dependent decrease in GLUT1 expression at 5 h, whereas at 24 h there was a down-regulation of both transporters (GLUT1 and GLUT4) in MM.1S cells[1].
8-Aminoadenosine inhibits cell proliferation, activated cell death, and does not activate transcription of the p53 target gene p21 or increase protein levels of either p53 or p21[1].
The toxic effects of 8-Aminoadenosine require adenosine kinase activity to convert 8-Aminoadenosine to 8-NH2-ATP in adenosine kinase-deficient cells[1].
Cell Viability Assay[1] | Cell Line: | MM.1S and U266 cells | | Concentration: | 0.1, 0.3, 1, 3, 10 μM | | Incubation Time: | For 48 hours | | Result: | Had IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively. |
Apoptosis Analysis[2] | Cell Line: | MCF-7 cells | | Concentration: | 10 μM | | Incubation Time: | For 24 hours | | Result: | Induced significant apoptotic death.
Apoptosis was not inhibited by knockdown of functional p53. |
Apoptosis Analysis[1] | Cell Line: | MM.1S cell line | | Concentration: | 3 μM | | Incubation Time: | 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 hours | | Result: | Induced the formation of LC3-II protein.
Caused the appearance of a population with a high AVO content with 1 μM for 24 hours. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
| 溶解性数据 | In Vitro: DMSO : 83.33 mg/mL(293.14 mM;Need ultrasonic) 配制储备液 | 1 mM | 3.5178 mL | 17.5889 mL | 35.1778 mL | | 5 mM | 0.7036 mL | 3.5178 mL | 7.0356 mL | | 10 mM | 0.3518 mL | 1.7589 mL | 3.5178 mL |
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