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Disitertide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:272105-42-7
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
P144
产品介绍
Disitertide (P144) 是转化生长因子TGFβ1的多肽抑制剂,特异性的阻断其与受体间的相互作用。Disitertide (P144) 也是PI3K的抑制剂和凋亡 (apoptosis) 诱导剂。
生物活性

Disitertide (P144) is a peptidictransforming growth factor-beta 1 (TGF-β1)inhibitor specifically designed to block the interaction with its receptor. Disitertide (P144) is also aPI3Kinhibitor and anapoptosisinducer[1][2][3][4][5].

体外研究
(In Vitro)

Disitertide (P144, 100 μg/mL) suppresses the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells[2].
Disitertide (TGF-β1 inhibitor) abrogates the MACC1- AS1 expression in GC cells, suggesting that targeting TGFβ signaling pathway may be a potential strategy to inhibit MSC-induced stemness and chemoresistance[3].
Disitertide (10 μg/mL to 200 μg/mL) affects proliferation, induces apoptosis as well as anoikis in A172 and U-87 MG GBM cell lines[5].

Western Blot Analysis[2]

Cell Line:Mouse embryo osteoblast precursor MC3T3-E1 cells
Concentration:100 μg/mL
Incubation Time:4 h
Result:Significantly suppressed the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells compared with the miR-590 group
体内研究
(In Vivo)

Disitertide (Topical application, 300 μg/mL) may promote scar maturation and improvement of hypertrophic scar morphology features in an “in vivo” model in nude mice after two weeks of treatment[4].

Animal Model:Human hypertrophic scars were implanted in 60 nude mice[4].
Dosage:300 μg/mL was added the Lipogel.
Administration:Topical application daily administered.
Result:Successful shedding was achieved in 83,3% of the xenografts.
Clinical Trial
分子量

1580.82

性状

Solid

Formula

C68H109N17O22S2

CAS 号

272105-42-7

Sequence Shortening

TSLDASIIWAMMQN

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-80°C2 years
-20°C1 year
In solvent-80°C6 months
-20°C1 month
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign value
Acidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1
Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1
Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetails
Negative (<0)1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
In Vivo:
  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (1.58 mM); Clear solution

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: 2.5 mg/mL (1.58 mM); Suspended solution; Need ultrasonic

  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (1.58 mM); Clear solution