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MOPIPP
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
MOPIPP图片
CAS NO:1485521-76-3
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
MOPIPP 是一种新型吲哚基查尔酮、液泡素-1,是一种 MOMIPP (HY-148114) 的非致命液泡诱导 2-丙基类似物。MOPIPP 能诱导细胞液泡化,增加自噬体数目。MOPIPP 还会引发细胞巨泡式死亡 (methuosis),并中断葡萄糖摄取和糖酵解代谢。MOPIPP 能够透过血脑屏障,对胶质母细胞瘤细胞有抑制作用。
生物活性

MOPIPP is a novel indolebased chalcone, and vacuolin-1, is a non-lethal vacuoleinducing 2-propyl analog ofMOMIPP(HY-148114). MOPIPP induces cellular vacuolization and increases autophagosomes numbers. MOPIPP also triggersmethuosis, and interrupts glucose uptake and glycolytic metabolism. MOPIPP can cross the blood-brain barrier and shows efficacy in suppressing tumor progression agaisnt glioblastoma cells[1][2][3].

体外研究
(In Vitro)

MOPIPP (10 μM; 48 h) induces cellular vacuolization but does not cause cell death in U251 glioblastoma cells, exhibiting characteristics of late endosomes[1][2].
MOPIPP (10 μM; 24 h) results an increasing LC3 fluorescence associated with the number of autophagosomes and inhibits fusion of autophagosomes with lysosomes[1].
MOPIPP (10 μM; 24 h) increases the amounts of exosomal marker proteins in vesicle fractions recovered from 293T cells[2].
MOMIPP (10 μM; 24 h and 5 h, respectively) causes early disruptions of glucose uptake and glycolytic metabolism, induces methuosis, a form of non-apoptotic cell death, in glioblastoma and other cancer cell lines[3].
MOMIPP (10 μM; 4 h and 24 h) selectively activates the JNK1/2 stress kinase pathway, resulting in phosphorylation of c-Jun, Bcl-2 and Bcl-xL[3].

Immunofluorescence[1]

Cell Line:U251 glioblastoma cells
Concentration:10 μM
Incubation Time:24 hours; incubated with 2.5 μg/mlAcridine Orange(HY-101879) for 45 min
Result:Caused accumulation of autophagosome markers.
Increased punctate LC3 fluorescence and suggested an increase in the number of autophagosomes in cells.

Western Blot Analysis[3]

Cell Line:U251 glioblastoma cells
Concentration:10 μM
Incubation Time:4 and 24 hours
Result:Triggered increaseing phosphorylation of Bcl-2 and Bcl-xL, accompanied the activation of JNK.
体内研究
(In Vivo)

MOMIPP (80 mg/kg; i.p.; single dose) readily penetrates the bloodbrain barrier in female Swiss Webster Mice and (80 mg/kg; i.p.; every 24 h; 15 d) is effective in suppressing progression of intracerebral glioblastoma xenografts in female NCR-Foxn1mice[3].

Animal Model:Intracerebral xenograft model in NCR-Foxn1mice (female, 7-8 weeks, injected with U251- LUC cells)[3]
Dosage:80 mg/kg
Administration:Intraperitoneal injection; every 24 hours for 15 days; monitored tumor progression by BLI on the days 7, 11, 15
Result:Significantly inhibited tumor progression.
分子量

320.39

Formula

C20H20N2O2

CAS 号

1485521-76-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.