| 包装 | 价格(元) |
| 10mg | 电议 |
| 50mg | 电议 |
Cell lines | Bovine embryos (IVPEs) |
Preparation Method | The effect of (DPPC (129Y83)-produced multilamellar vesicles (MLVs) on the lipid profile of the plasma membrane of IVP bovine embryos was also evaluated by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). The MLVs were prepared by the lipid hydration method. Briefly, a DPPC chloroform solution (10-3 mol/L) was placed in a glass vial, followed by the chloroform evaporation under nitrogen flow. The MLVs were prepared by adding the appropriate aqueous solution to the DPPC film containing vial at 55℃, stirred for 1 h before cooling down to room temperature. MLV stability was investigated by measuring the vesicle size with different media and time intervals. The film was resuspended in ultrapure water and SOFaaci medium in the absence or presence of 2.5% foetal bovine serum (FBS; v/v), 5 mg/mL bovine serum albumin (BSA), and 13 mmol/L pyruvate. For each resuspension medium, three different lipid concentrations were used: 1.0, 1.5, or 2.0 mmol/L. In addition, the MLVs were evaluated immediately after production (refrigerated at 4℃) and after incubation at 38.5℃ in a controlled gas atmosphere (5% O2, 5% CO2, and 90% N2) for 24 and 48 h. |
Reaction Conditions | 1.0 and 1.5 mmol/L DPPC (129Y83) for 24 h |
Applications | The largest MLVs were observed with 1.0 and 1.5 mmol/L DPPC (129Y83) (with or without incubation) in water compared with SOFaaci medium. After 24 h of incubation, MLVs with the largest diameter (approximately 1.5 μm) were obtained with 1.0 mmol/L DPPC (129Y83) in the culture medium. |
Animal models | Female BALB/c mice (6-wk-old) |
Preparation Method | Liposomes for liposome immune lysis assay (LILA) were prepared from a lipid mixture solution containing DMPC (0.5 μmol), Chol (0.5 μmol), DPPA (0.05 μmol) and GSLs (0.05 μmol) and used for detection of anti-GSL antibodies.Mice was intraperitoneally immunized as follows: group I, GSL alone (0.05 μmol); group II, liposome composed of DMPC, Chol , S. minnesota R595 LPS and GSL; group III, liposome composed of DPPC (129Y83) (0.5 μmol), Chol , S. minnesota R595 LPS and GSL (DPPC (129Y83)-liposome); group IV, liposome composed of DSPC, Chol, and S. minnesota R595 LPS and GSL. All were immunized with 0.5 ml of the immunogen per mouse. |
Dosage form | 0.05 μmol DPPC (129Y83) |
Applications | DPPC (129Y83)-liposome would serve effectively as a delivery vehicle for inducing immune responses against GSL antigen. |
| 产品描述 | DPPC (129Y83) is a phosphoglyceride that can be used in liposome preparation. When to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC (129Y83)) in co-culture with in vitro-produced bovine embryos (IVPEs). Incubation (24 and 48 h) did not impair the MLV structure but affected the average diameter. The rate of blastocyst production was not reduced, demonstrating the nontoxicity of the MLVs even at 2.0 mmol/L[1]. The interactions in cholesterol/DPPC (129Y83)/APC films were found to be weaker than those in the cholesterol/DPPC (129Y83) system, serving as a model of healthy cell membranes, thus proving that the incorporation of APCs is, from a thermodynamic point of view, unfavorable for binary cholesterol/DPPC (129Y83) monolayers[2]. To elucidate the interaction of a cationic surfactant synthesized, investigations involving Nα-benzoyl-arginine decyl amide (Bz-Arg-NHC10), and model membranes composed by 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC (129Y83)) were done. As the concentration of Bz-Arg-NHC10 increased, the main transition temperature of DPPC (129Y83) slightly decreased[3]. MPOx copolymers are proved to modify both the size and lamellarity of DPPC (129Y83) liposomes. The gradient copolymer with higher hydrophilic content induces shrinkage of the uni- and bi-lamellar DPPC (129Y83) vesicles[4]. DPPC (129Y83) liposomes enhanced the pulmonary absorption of unencapsulated free insulin;although unencapsulated free insulin spreads throughout the alveolar mucus layer, the concentration of insulin released near the absorption surface is increased by the encapsulation of insulin into DPPC (129Y83) liposomes and the absorption efficiency is also increased[5]. When show how oppositely charged gold nanoparticles (Au-NPs) interact with monolayers of the zwitterionic DPPC (129Y83). For the zwitterionic DPPC (129Y83), on the other hand, significant effects only occurred for negatively charged NPs, including a decrease in elasticity[6]. DPPC (129Y83)-liposome would serve effectively as a delivery vehicle for inducing immune responses against GSL antigen[7]. References: |
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